Oxygen reduction reactions of the thermostable bilirubin oxidase from Bacillus pumilus on mesoporous carbon-cryogel electrodes
Oxygen reduction reactions of the thermostable bilirubin oxidase from Bacillus pumilus on mesoporous carbon-cryogel electrodes
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DOI：
10.1016/j.electacta.2013.11.131
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发表时间：
2014-01
影响因子：
6.6
通讯作者：
S. Tsujimura;Emmanuel Suraniti;F. Durand;N. Mano
中科院分区：
文献类型：
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作者：
S. Tsujimura;Emmanuel Suraniti;F. Durand;N. Mano
本研究展示了一种新的胆红素氧化酶（BOD）从短小芽孢杆菌的介孔碳冻凝胶（CCG）电极上的生物电催化反应，在存在和不存在的介体。物理吸附在CCG电极的介孔基质上的BOD允许从碳电极到酶的I型铜位点的直接电子转移（DET）。由BOD催化的分子氧还原反应（ORR）的电流依赖于电解质的温度和pH。还使用锇基氧化还原聚合物研究了BOD在CCG电极上催化的介导的ORR。用0.2 mg cm− 2水凝胶修饰的CCG电极上的催化电流为1.8 mA cm−2，该水凝胶由酶、氧化还原聚合物和交联剂组成，对于相同的水凝胶组成和负载，该电流几乎是平坦玻璃碳电极上的催化电流的5倍。多孔碳电极上的催化电流与水凝胶的总量呈线性关系，而平板电极上的催化电流与负载量无关。
This study demonstrates the bioelectrocatalytic reactions of a new bilirubin oxidase (BOD) from Bacillus pumilus on a mesoporous carbon cryogel (CCG) electrode, in the presence and absence of a mediator. BOD, physically adsorbed on the mesoporous matrix of a CCG electrode, allowed a direct electron transfer (DET) from the carbon electrode to the type I copper site of the enzyme. The current from the dioxygen reduction reaction (ORR), catalyzed by BOD, depended on the temperature and pH of the electrolyte. The mediated ORR catalyzed by BOD on CCG electrode was also investigated using osmium based redox polymers. The catalytic current on the CCG electrode modified with 0.2 mg cm−2of hydrogel consisting of an enzyme, a redox polymer and a cross linker, was 1.8 mA cm−2, which was almost five times higher than that on a flat glassy carbon electrode for the same hydrogel composition and loading. The catalytic current linearly increased with the total amount of hydrogel on the porous carbon electrode while the catalytic current on the flat electrode was indifferent to the loading.