LIPIDAT - A DATABASE OF LIPID PHASE-TRANSITION TEMPERATURES AND ENTHALPY CHANGES - DMPC DATA SUBSET ANALYSIS
LIPIDAT - A DATABASE OF LIPID PHASE-TRANSITION TEMPERATURES AND ENTHALPY CHANGES - DMPC DATA SUBSET ANALYSIS
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DOI：
10.1016/0009-3084(92)90002-7
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发表时间：
1992-03-01
影响因子：
3.4
通讯作者：
HOGAN, J
中科院分区：
文献类型：
--
作者：
CAFFREY, M;HOGAN, J
大约30年前，对合成和生物衍生的脂质的介形期性质的系统研究开始。在过去的十年中，对这一领域的兴趣已大大增长。结果，存在有关脂质阶段行为的大量信息，但是不幸的是，这些数据到现在已经散布在各种书籍，会议记录和期刊上。这些数据最近是在集中式数据库Lipidat中编译的，以期提供现成的数据和适当文献的访问。 Lipidat包括所有已知的介生和多态相变温度的制表，以及在干燥，部分和部分水合状态下合成和生物学衍生的脂质的焓变。还包括pH，盐和金属离子浓度以及其他添加剂（例如蛋白质，药物等）对热力学值的影响。报告了用于进行测量和实验条件的方法。书目信息包括全面的文献参考和作者名单，但目前不包括文章标题。截至撰写本文时，数据库一直是当前截至1990年6月，并且已接近10 000条记录。每个记录包含28个字段。在本文中，我们报告了含量并提出了脂质分析，因为它是指完全水合的1,2-二吡啶烷-SN-甘油-3-磷脂（DMPC）。该数据库子集约占所有Lipidat记录的7％。它包括从1967年到1989年的23年期间收集的数据，由702个记录组成，这些记录是从55种不同期刊中的336篇文章中获得的。从197 1开始，每年的记录数量稳步上升，在1977年最多达到89张记录，并且在成功期间以60-70的记录/年保持相对恒定。对DMPC子集做出最大贡献的期刊包括Biochimica et Biophysica Acta，生物化学，脂质的化学和物理学和生物物理学杂志。这四个期刊占数据库子集中总记录的71％。分析表明，差异扫描量热法，电子自旋共振，荧光，核磁共振和拉曼光谱是最常用的DMPC过渡温度测定方法。关于及时的地方，不同的方法假设或宽松的流行，就出现了一个有趣的模式。在文献中，分别描述了25、9和2个不同的方式，这一观察结果表明迫切需要普遍接受的脂质相表示法。提出了在加热和冷却方向上确定的主要和前过渡温度和焓的分布，以及它们对所使用的水相添加，扫描速率和方法的依赖。
The systematic study of the mesomorphic phase properties of synthetic and biologically derived lipids began some 30 years ago. In the past decade, interest in this area has grown enormously. As a result, there exists a wealth of information on lipid phase behavior, but unfortunately these data have until now been scattered throughout the literature in a variety of books, proceedings and journals. The data have recently been compiled in a centralized database, LIPIDAT, with a view to providing ready access to the data and to the appropriate literature. LIPIDAT consists of a tabulation of all known mesomorphic and polymorphic phase transition temperatures and enthalpy changes for synthetic and biologically-derived lipids in the dry and in the partially and fully hydrated states. Also included is the effect of pH, and of salt and metal ion concentration and other additives such as proteins, drugs, etc., on the thermodynamic values. The methods used in making the measurements and the experimental conditions are reported. Bibliographic infomation includes comprehensive literature referencing and list of authors, but does not at the present time include article titles. As of this writing, the database is current through June, 1990 and is approaching 10 000 records in length. Each record contains 28 fields. In this paper we report the contents and present an analysis of LIPIDAT as it refers to fully hydrated 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). This database subset represents about 7% of all LIPIDAT records. It includes data collected over a 23-year period from 1967 to 1989 and consists of 702 records obtained from 336 articles in 55 different journals. The number of records per year rises steadily beginning in 197 1, reaches a maximum of 89 records/year in 1977 and remains relatively constant at 60-70 records/year in the succeeding period. Journals making the greatest contribution to the DMPC subset include Biochimica et Biophysica Acta, Biochemistry, Chemistry and Physics of Lipids and the Biophysical Journal. These four journals account for 71% of the total records in the database subset. The analysis shows that differential scanning calorimetry, electron spin resonance, fluorescence, nuclear magnetic resonance and Raman spectroscopy are the methods most commonly used for DMPC transition temperature determination. An interesting pattern emerges as to the place in time the different methods assume or loose popularity. The main, pre- and subtransition have been described, respectively, in 25, 9 and 2 distinct ways in the literature, an observation that points to the urgent need for a universally accepted lipid phase notation. The distribution of main and pre-transition temperatures and enthalpies determined in the heating and cooling direction and their dependence on aqueous phase additive, scan rate and methods used is presented.