Epithelial clara cell injury occurs in bronchiolitis obliterans syndrome after human lung transplantation.
Epithelial clara cell injury occurs in bronchiolitis obliterans syndrome after human lung transplantation.
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DOI：
10.1111/j.1600-6143.2012.04201.x
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Palmer SM
中科院分区：
文献类型：
--
作者：
Kelly FL;Kennedy VE;Jain R;Sindhwani NS;Finlen Copeland CA;Snyder LD;Eu JP;Meltzer EB;Brockway BL;Pavlisko E;Stripp BR;Palmer SM
闭塞性细支气管炎综合征（BOS）是一种进行性气流阻塞的疾病，影响大多数肺移植受者并限制移植后的长期生存。虽然上皮损伤似乎是BOS发展的核心，但关于在这种情况下受影响的特定上皮细胞类型知之甚少。我们假设BOS会优先损伤分泌型Clara细胞，这些细胞在先天防御和上皮修复中发挥作用。为了验证这一假设，我们评估了组织转录，组织蛋白，和肺液蛋白表达的克拉拉细胞分泌蛋白（CCSP），克拉拉细胞的标志物，在肺移植受者与BOS，无BOS患者，并在供体对照。我们的研究结果表明，CCSP组织转录和蛋白质表达显着减少，在肺移植受者与BOS相比，无BOS或供体对照。此外，我们证明，CCSP蛋白水平显着降低与BOS患者的肺液相比，无BOS的控制，在横截面和纵向分析。总的来说，这些互补的结果表明，BOS涉及细支气管Clara细胞的分布和功能的选择性改变。
Bronchiolitis obliterans syndrome (BOS) is a condition of progressive airflow obstruction that affects a majority of lung transplant recipients and limits long-term post-transplant survival. Although epithelial injury appears central to the development of BOS, little is known regarding the specific epithelial cell types that are affected in this condition. We hypothesized that BOS would involve preferential injury to the secretory Clara cells that function in innate defense and epithelial repair. To test this hypothesis, we assessed tissue transcript, tissue protein, and lung fluid protein expression of Clara cell secretory protein (CCSP), a marker for Clara cells, in lung transplant recipients with BOS, BOS-free patients, and in donor controls. Our results demonstrate that CCSP tissue transcript and protein expression are significantly reduced in lung transplant recipients with BOS as compared to BOS-free or donor controls. In addition, we demonstrate that CCSP protein levels are significantly reduced in the lung fluid of patients with BOS as compared to BOS-free controls, in cross-sectional and longitudinal analysis. Collectively, these complementary results illustrate that BOS involves a selective alteration in the distribution and function of bronchiolar Clara cells.
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影响因子：
6.2
作者：
Palmer, SM;Davis, RD;Reinsmoen, NL
通讯作者：
Reinsmoen, NL
影响因子：
6.2
作者：
Mattsson, J;Remberger, M;Nord, M
通讯作者：
Nord, M
影响因子：
15.9
作者：
Burlingham, William J.;Love, Robert B.;Willkes, David S.
通讯作者：
Willkes, David S.
DOI：
10.1164/rccm.200408-1129oc
发表时间：
2005-04-01
影响因子：
24.7
作者：
Palmer, SM;Burch, LH;Schwartz, DA
通讯作者：
Schwartz, DA
影响因子：
6.2
作者：
Wolf, Thomas;Oumeraci, Tonio;von Neuhoff, Nils
通讯作者：
von Neuhoff, Nils