In vivo bone regeneration with injectable calcium phosphate biomaterial:: A three-dimensional micro-computed tomographic, biomechanical and SEM study
In vivo bone regeneration with injectable calcium phosphate biomaterial:: A three-dimensional micro-computed tomographic, biomechanical and SEM study
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DOI：
10.1016/j.biomaterials.2005.01.072
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Daculsi, G
中科院分区：
文献类型：
--
作者：
Gauthier, O;Müller, R;Daculsi, G
这项体内研究通过无损三维 (3D) 显微断层扫描 (mu CT) 成像、采用无损微压痕技术的生物力学测试和 2D 扫描电子显微镜 (SEM) 分析，研究了所有可注射磷酸钙骨替代品 (IBS) 在骨再生过程中的效率。可注射生物材料是通过混合双相磷酸钙（BCP）陶瓷矿物相和纤维素聚合物获得的。 BCP颗粒的直径为200-500μm或80-200μm。将可注射材料植入兔子股骨远端的临界尺寸骨缺损中，持续 6 周。2D 和 3D 技术均注意到广泛的新骨沉积。 Micro-CT显示新形成的骨与小梁宿主骨结构完美连续，并证明了恢复的骨网络的高度互连性。对于两种 IBS 制剂，SEM 和 mu CT 给出了非常接近的测量结果。唯一检测到的显着差异涉及 IBS 80-200 获得的新形成骨的数量，该数量在 mu CT 分析中明显高于 SEM (p = 0.00007)。学生 t 检验没有显示新形成的骨量和从 mu CT 分析或 SEM 获得的剩余陶瓷量有任何显着差异。回归分析显示，从 mu CT 或 SEM 获得的新形成骨量和剩余陶瓷量之间存在令人满意的相关性。对于IBS 200-500，SEM显示缺损内新形成的骨率为28.0 +/- 5.2%，样品的屈服强度为18.8 +/- 5.4 MPa。对于IBS 80-200，SEM显示缺损内新形成的骨率为31.7 +/- 5.1 %，样品的屈服强度为26.8 +/- 4.5 Wa。屈服强度似乎与新形成的骨量密切相关，特别是通过 mu CT 观察到的。这项研究表明，无损技术能够研究可注射生物材料替代骨的生物和机械方面。 (c) 2005 Elsevier Ltd. 保留所有权利。
This in vivo study investigated the efficiency of all injectable calcium phosphate bone substitute (IBS) for bone regenerative procedures through non-destructive three-dimensional (3D) micro-tomographic (mu CT) imaging, biomechanical testing with a nondestructive micro-indentation technique and 2D scanning electron microscopy (SEM) analysis. The injectable biomaterial was obtained by mixing a biphasic calcium phosphate (BCP) ceramic mineral phase and a cellulosic polymer. The BCP particles were 200-500 mu m or 80-200 mu m in diameter. The injectable material was implanted for 6 weeks into critical-sized bone defects Lit the distal end of rabbit femurs.Extensive new bone apposition was noted with both 2D and 3D techniques. Micro-CT showed that newly formed bone was in perfect continuity with the trabecular host bone structure and demonstrated the high interconnectivity of the restored bone network. For both IBS formulations, SEM and mu CT gave very close measurements. The only detected significant difference concerned the amount of newly formed bone obtained with IBS 80-200 that appeared significantly higher with mu CT analysis than with SEM (p = 0.00007). Student t-tests did not show any significant difference in the amount of newly formed bone and remaining ceramic obtained from mu CT analysis or SEM. Regression analysis showed satisfactory correlation between both the amount of newly formed bone and remaining ceramic obtained from mu CT or SEM. For IBS 200-500, the newly formed bone rate inside the defect was 28.0 +/- 5.2% with SEM and yield strength of the samples was 18.8 +/- 5.4 MPa. For IBS 80-200, the newly formed bone rate inside the defect was 31.7 +/- 5.1 % with SEM and yield strength of the samples was 26.8 +/- 4.5 Wa. Yield strength appeared well correlated with the amount of newly formed bone, specially observed with mu CT.This study showed the ability of non-destructive techniques to investigate biological and mechanical aspects of bone replacement with injectable biomaterials. (c) 2005 Elsevier Ltd. All rights reserved.