Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening
Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening
复制标题
Src SH2结构域的合成及其在镜像筛选生物测定中的应用
DOI：
10.1039/c7ra07445j
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发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Oishi Shinya
中科院分区：
文献类型：
--
作者：
Shu Keitou;Noguchi Taro;Honda Kaori;Kondoh Yasumitsu;Osada Hiroyuki;Ohno Hiroaki;Fujii Nobutaka;Oishi Shinya
Src SH 2结构域通过两个片段肽的天然化学连接来合成。简单的方案用于制备镜像SH 2结构域（D-Src SH 2结构域）和四甲基罗丹明标记的SH 2结构域。合成的SH 2结构域被正确折叠并显示出活性，使用这些蛋白质，我们建立了生物测定，以确定新的Src SH 2结构域抑制剂从一个未开发的镜像库的天然产物。
The Src SH2 domain was synthesized via native chemical ligation of two fragment peptides. The facile protocol was used to prepare the mirror-image SH2 domain (D-Src SH2 domain) and tetramethylrhodamine-labeled SH2 domains. The synthesized SH2 domains were correctly folded and showed activity, and using these proteins we established bioassays to identify novel Src SH2 domain inhibitors from an unexplored mirror-image library of natural products.