Combination of retinoids and narrow-band ultraviolet B inhibits matrix metalloproteinase 13 expression in HaCaT keratinocytes and a mouse model of psoriasis.
Combination of retinoids and narrow-band ultraviolet B inhibits matrix metalloproteinase 13 expression in HaCaT keratinocytes and a mouse model of psoriasis.
复制标题
类视黄醇和窄带紫外线 B 的组合可抑制 HaCaT 角质形成细胞和银屑病小鼠模型中基质金属蛋白酶 13 的表达
DOI：
10.1038/s41598-021-92599-w
复制
发表时间：
2021-06-25
影响因子：
4.6
通讯作者：
Luo S
中科院分区：
文献类型：
--
作者：
Xi C;Xiong C;Wang H;Liu Y;Luo S
基质金属蛋白酶13(MMP13)可由角质形成细胞和成纤维细胞释放，参与皮肤病的发病机制。维甲酸衍生物药物包括他扎罗汀和阿维A。他扎罗汀/阿维A和窄谱紫外线B(NB-UVB)照射是治疗银屑病的常见选择。然而，在银屑病的背景下，它们对MMP13表达的影响还有待确定。检测银屑病患者外周血中MMP13的表达。我们还观察了他扎罗汀/阿维A和NB-UVB对银屑病小鼠模型MMP13表达的影响。将人HaCaT角质形成细胞暴露于阿维A或NB-UVB，然后检测细胞增殖和MMP13表达水平。我们发现银屑病患者皮损和血清中MMP13蛋白水平升高。阿维A和NB-UVB单独或联合照射可降低HaCaT细胞的增殖和MMP13的表达。在小鼠模型中，他扎罗汀治疗或NB-UVB照射一致地减轻了咪喹莫特诱导的牛皮癣样皮炎并减少了MMP13的表达。在HaCaT角质形成细胞和动物实验的基础上，我们认为他扎罗汀/阿维A和NB-UVB照射可以抑制HaCaT角质形成细胞和银屑病小鼠模型MMP13的表达。阻断MMP13活性可能在改善银屑病症状方面具有治疗潜力。
Matrix metalloproteinase13 (MMP13) can be released by keratinocytes and fibroblasts and involved in the pathogenesis of skin disorders. Retinoic acid derivative drugs include tazarotene and acitretin. Tazarotene/acitretin and narrow-band ultraviolet B (NB-UVB) irradiation are common treatment options for psoriasis. However, their impact on MMP13 expression in the context of psoriasis has yet to be determined. The expression of MMP13 was analyzed in patients with psoriasis. The effects of tazarotene/acitretin and NB-UVB on MMP13 expression were also investigated in a mouse model of psoriasis. Human HaCaT keratinocytes were exposed to acitretin or NB-UVB and then assayed for cell proliferation and MMP13 expression levels. We showed that patients with psoriasis had increased levels of MMP13 protein in skin lesions and serum samples. Exposure to acitretin and NB-UVB irradiation alone or in combination led to reduction of cell proliferation and MMP13 expression in HaCaT cells. Consistently, tazarotene treatment or NB-UVB irradiation attenuated imiquimod-induced psoriasis-like dermatitis and decreased MMP13 expression in a mouse model. Based on these from HaCaT keratinocytes cells and animal experiments, we suggest that tazarotene/acitretin and NB-UVB irradiation can inhibit the expression of MMP13 in HaCaT keratinocytes and psoriasis mouse models. Blockade of MMP13 activity may have therapeutic potential in improving symptoms of psoriasis.
登录
查看更多内容
影响因子：
27.4
作者：
van Kuijk AW;Gerlag DM;Vos K;Wolbink G;de Groot M;de Rie MA;Zwinderman AH;Dijkmans BA;Tak PP
通讯作者：
Tak PP
影响因子：
5.6
作者：
Diani, Marco;Perego, Silvia;Lombardi, Giovanni
通讯作者：
Lombardi, Giovanni
DOI：
10.4103/0378-6323.72461
发表时间：
2010-11-01
影响因子：
2.9
作者：
Dogra, Sunil;De, Dipankar
通讯作者：
De, Dipankar
影响因子：
3
作者：
Vasku, Vladimir;Vasku, Julie Bienertova;Vasku, Anna
通讯作者：
Vasku, Anna
MicroRNA-210过表达通过诱导Th1和Th17细胞分化促进银屑病样炎症
影响因子：
15.9
作者：
Wu, Ruifang;Zeng, Jinrong;Lu, Qianjin
通讯作者：
Lu, Qianjin