Proanthocyanidins alter adhesive/dentin bonding strengths when included in a bonding system.
Proanthocyanidins alter adhesive/dentin bonding strengths when included in a bonding system.
复制标题
DOI：
--
复制
发表时间：
2012-10
影响因子：
1.4
通讯作者：
B. Hechler;X. Yao;Yong Wang
中科院分区：
文献类型：
--
作者：
B. Hechler;X. Yao;Yong Wang
目的研究原花青素（PA）加入黏合体系对缓冲剂和胶原酶长期保存后牙本质/粘接剂黏合稳定性的影响。方法采用无PA （0-PA）、PA加入引物（PA-引物）、PA加入胶粘剂（PA-adhesive）三种方式粘接人牙本质表面，形成复合堆积物。切片成梁后，粘合试样在缓冲液或胶原酶中保存0、1、4、26或52周，然后进行微拉伸粘结强度（muTBS）测试。方差分析和Tukey’s HSD事后分析。用扫描电子显微镜（SEM）观察断裂表面。结果粘接系统和储存时间对muTBS有显著影响，而储存介质对muTBS无显著影响。最初，0-PA组和PA-引物组均优于PA-胶粘剂组，1周后，两组均低于0-PA组。但4周后PA-adhesive显著升高，0-PA显著降低，三组均相同。此后，两个pa -引物/粘合剂组都有增加的趋势（0-PA组保持一致），因此在52周时，与0-PA样品相比，pa -引物样品明显更强（P < 0.001）或接近（P = 0.08）。扫描电镜显示，0-PA和pa -引物组的初始断裂倾向于发生在杂化层的中间/底部，而pa -粘合剂的初始断裂倾向于发生在杂化层的顶部/粘合剂中。然而，在4周后，所有组在杂交层的中间/底部发生相似的断裂。
PURPOSE To determine the effect of proanthocyanidins (PA) incorporation into a bonding system on dentin/adhesive bond stability following long-term storage in buffer and collagenase. METHODS Human dentin surfaces were bonded with no PA (0-PA), PA incorporated in the primer (PA-primer), or PA incorporated in the adhesive (PA-adhesive), and composite build-ups were created. Following sectioning into beams, bonded specimens were stored in buffer or collagenase for 0, 1, 4, 26, or 52 weeks before being tested for microtensile bond strength (muTBS). ANOVA and Tukey's HSD post-hoc were performed. Fractured surfaces were viewed with scanning electron microscopy (SEM). RESULTS Both bonding system and storage time but not storage medium significantly affected muTBS. Initially, 0-PA and PA-primer were superior to PA-adhesive, and after 1 week both PA groups were inferior to 0-PA. However, after 4 weeks PA-adhesive had significantly increased and 0-PA significantly decreased such that all three groups were equal. Thereafter, both PA-primer/adhesive groups trended with an increase (the 0-PA group remaining consistent) such that at 52 weeks PA-primer samples were significantly stronger (P < 0.001) or nearly so (P = 0.08) when compared to 0-PA samples. SEM revealed that initial fractures tended to occur at the middle/bottom of the hybrid layer for 0-PA and PA-primer groups but at the top of the hybrid layer/in the adhesive for PA-adhesive. After 4 weeks, however, all groups fractured similarly at the middle/bottom of the hybrid layer.