Synthetic Biology of Small RNAs and Riboswitches.
Synthetic Biology of Small RNAs and Riboswitches.
复制标题
小RNA和核糖开关的合成生物学。
DOI：
10.1128/microbiolspec.rwr-0007-2017
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发表时间：
2018-05
影响因子：
3.7
通讯作者：
Hammond MC
中科院分区：
文献类型：
--
作者：
Villa JK;Su Y;Contreras LM;Hammond MC
在细菌和古菌中，小RNA(SRNAs)通过伴侣蛋白介导的与靶mRNAs的反义相互作用来调节复杂的网络，而核糖开关则基于结合小分子配体的能力来调节顺式基因的表达。尽管我们对这两个重要的调控RNA类的理解和表征还远未完成，但这些基于RNA的机制已被证明在合成生物学的广泛应用中是有用的。除了在代谢工程和正交基因控制领域的经典和当代应用外，本综述还包括调控RNA作为生物传感器、逻辑门和确定RNA-RNA相互作用的工具的最新应用。另有一节侧重于对sRNA和核糖开关的基础研究所获得的关键见解和提出的挑战，这些研究应有助于合成调控RNA的未来发展。
In bacteria and archaea, small RNAs (sRNAs) regulate complex networks through antisense interactions with target mRNAs mediated by chaperone proteins, and riboswitches regulate gene expression in cis based on the ability to bind small molecule ligands. Although our understanding and characterization of these two important regulatory RNA classes is far from complete, these RNA-based mechanisms have proven useful for a wide variety of synthetic biology applications. Besides classic and contemporary applications in the realm of metabolic engineering and orthogonal gene control, this review also covers newer applications of regulatory RNAs as biosensors, logic gates, and tools to determine RNA-RNA interactions. A separate section focuses on critical insights gained and challenges posed by fundamental studies of sRNAs and riboswitches that should aid future development of synthetic regulatory RNAs.