[Laboratory-performance study of quantitative PCR methods to analyze an approved genetically modified maize (Mon810 Line)].
[Laboratory-performance study of quantitative PCR methods to analyze an approved genetically modified maize (Mon810 Line)].
复制标题
[用于分析已批准的转基因玉米（Mon810 系）的定量 PCR 方法的实验室性能研究]。
DOI：
10.3358/shokueishi.47.15
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
T. Maitani
中科院分区：
文献类型：
--
作者：
Takahiro Watanabe;K. Kasama;Hiroyuki Kikuchi;Tatsuya Suzuki;S. Tokishita;Kozue Sakata;A. Matsuki;A. Hino;H. Akiyama;T. Maitani
进行了实验室性能研究，以调查定量PCR方法分析批准的转基因（GM）玉米（Mon 810系）的可靠性的影响因素。将测试玉米粉末样品制备为含有高浓度（指定值; 5.45%）或低浓度（指定值; 0.35%）Mon 810系的盲样。在确认其均一性后，将其提供给参与合作研究的27个实验室。从所有实验室收集数据并进行统计分析。两个使用Roche LightCycler（LC）的实验室报告了显著较高的检测值。进一步检查发现，LC法受设备本身或PCR试剂的影响较大，重复性较差。另一方面，一些使用ABI定量PCR设备的实验室报告了错误的检测值。在这些实验室中，错误似乎是由于DNA的质量和/或产量不足。为了确定影响测试值的因素，分析分类群特异性基因的测量值将是有用的。此外，改进的硅胶膜DNA提取方法使得可以比以前更容易地在更短的时间内提取所需量的DNA。
A laboratory-performance study was carried out to investigate factors affecting the reliability of the quantitative PCR method to analyze an approved genetically modified (GM) maize (Mon810 line). Test maize powdered samples were prepared as blind samples containing a high (assigned value; 5.45%) or low (assigned value; 0.35%) concentration of the Mon810 line. After confirmation of their homogeneity, they were provided to 27 laboratories participating in the collaborative study. The data were collected from all laboratories and statistically analyzed. Two laboratories, which used a Roche LightCycler (LC), reported significantly high test values. A further examination showed that the LC method is greatly affected by the equipment itself or PCR reagents, resulting in poor repeatability. On the other hand, some laboratories, which used ABI quantitative PCR equipment, reported erroneous test values. In these laboratories, the errors appeared to have been due to inadequate quality and/or yield of DNA. To identify factors affecting the test values, analysis of the measured values for the taxon-specific gene will be useful. Furthermore, the modified silica-gel membrane DNA extraction method made it possible to extract the required amounts of DNA more easily and in a shorter time than before.
AOAC 2009.01 使用基于官方酶-HPLC 方法的改良方法测定加工食品中难消化低聚糖含量的准确性
DOI：
--
发表时间：
2014
期刊：
影响因子：
--
作者：
岩本綾香;古瀬充宏;山崎いづみ;安尾しのぶ;田辺賢一，中村禎子，大曲勝久，奥 恒行
通讯作者：
田辺賢一，中村禎子，大曲勝久，奥 恒行
影响因子：
1.5
作者：
Hino, A
通讯作者：
Hino, A