Effects of resveratrol on autophagy and the expression of inflammasomes in a placental trophoblast oxidative stress model
Effects of resveratrol on autophagy and the expression of inflammasomes in a placental trophoblast oxidative stress model
复制标题
白藜芦醇对胎盘滋养层氧化应激模型自噬和炎症小体表达的影响
DOI：
10.1016/j.lfs.2020.117890
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发表时间：
2020-09-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Runxia
中科院分区：
文献类型：
--
作者：
Li, Meihe;Wu, Xiaoling;Liu, Runxia
目的：探讨滋养细胞氧化应激模型中是否存在NLRP 1的激活和自噬。方法：HTR-8/SVneo细胞经H2 O2处理3 h后，检测细胞内ROS水平及细胞凋亡情况。检测IL-1 β、caspase-1、NLRP 1、LC 3和Beclin-1的水平。结果：300 μ mol/L H2 O2作用3 h是建立氧化应激损伤模型的最佳组合（P < 0.01）。LDH、ROS、MDA含量升高，SOD、CAT活性降低（P < 0.01）。细胞凋亡率增加（P < 0.01）。IL-1 β、caspase-1、NLRP 1、LC 3和Beclin-1蛋白表达均高于正常对照组（P
Objective: We aim to investigate whether there is activation of NLRP1 and autophagy in trophoblast oxidative stress model. Resveratrol was taken to clarify its role in oxidative damage of placental trophoblasts.Methods: H2O2 was added to HTR-8/SVneo cell for 3 h, then the ROS level and apoptosis panel was performed. The levels of IL-1 beta, caspase-1, NLRP1, LC3 and Beclin-1 were detected. Resveratrol was added after 8 h, the ROS level and apoptosis rate were detected, the expression of IL-1 beta, caspase-1, NLRP1, LC3 and Beclin-1 were detected.Results: 300 mu mol/L H2O2 for 3 h is the optimum combination in establishing the oxidative stress injury model (P < 0.01). LDH, ROS and MDA level was increased, the activity of SOD, CAT were declined (P < 0.01). Apoptosis rate increased (P < 0.01). The expression of IL-1 beta, caspase-1, NLRP1, LC3 and Beclin-1 protein was higher (P