Down-regulation of BTG3 promotes cell proliferation, migration and invasion and predicts survival in gastric cancer
Down-regulation of BTG3 promotes cell proliferation, migration and invasion and predicts survival in gastric cancer
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BTG3 的下调促进细胞增殖、迁移和侵袭并预测胃癌的生存
DOI：
10.1007/s00432-014-1826-9
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发表时间：
2015-03-01
影响因子：
3.6
通讯作者：
Liang, L.
中科院分区：
文献类型：
--
作者：
Ren, X. L.;Zhu, X. H.;Liang, L.
胃癌（Gastric cancer，GC）是我国最常见的恶性肿瘤之一。B-cell translocation gene 3（BTG3）在多种肿瘤中被鉴定为肿瘤抑制基因，但其在GC中的作用尚不清楚。本研究旨在检测BTG 3在GC组织中的表达及其预后价值，并确定其在GC进展中的作用。方法采用实时RT-PCR、Western印迹或免疫组织化学方法检测BTG 3在GC细胞系和组织中的表达。通过一系列体内外实验，观察BTG3对胃癌细胞增殖、迁移和侵袭能力的影响。其表达与远处转移呈正相关（P <0.05）。BTG3表达较低的患者总生存时间较短（P = 0.015）。BTG3在体外和体内均能抑制胃癌细胞的增殖。结论BTG3表达下调与胃癌细胞的增殖、迁移和侵袭密切相关。它可能是胃癌患者的一种新的预后生物标志物。
BackgroundGastric cancer (GC) is one of the most common malignancies in China. B-cell translocation gene 3 (BTG3) has been identified as a tumor suppressor in several tumors, but its role in GC remains unknown. This study aimed to detect the expression of BTG3 and its prognostic value in GC tissues and determine its function in the progression of GC.MethodologyThe expression of BTG3 was detected in GC cell lines and tissues by real-time RT-PCR, Western blot or immunohistochemistry. A series of in vitro and in vivo assays were performed to evaluate the effect of BTG3 on proliferation, migration and invasion of GC cells.ResultsB-cell translocation gene 3 was obviously down-regulated in GC tissues. Its expression was positively correlated with distant metastasis (P< 0.05). Patients with lower BTG3 expression had shorter overall survival time (P= 0.015). BTG3 suppressed the proliferation of GC cells in vitroandin vivo. It also inhibited migration and invasion of GC cells in vitro.ConclusionDown-regulation of BTG3 is closely associated with proliferation, migration and invasion in GC. It may be a novel prognostic biomarker for GC patients.