Imaging Switchable Protein Interactions with an Active Porous Polymer Support
Imaging Switchable Protein Interactions with an Active Porous Polymer Support
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DOI：
10.1021/acs.jpcb.0c01807
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发表时间：
2020-06-04
影响因子：
3.3
通讯作者：
Landes, Christy F.
中科院分区：
文献类型：
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作者：
Dutta, Chayan;Bishop, Logan D. C.;Landes, Christy F.
关于多孔界面的局部物理化学如何驱动蛋白质运输机制的机制细节对于优化生物材料应用是必要的。由刺激响应聚合物制成的交联水凝胶具有通过可调空间和化学转化捕获和释放活性蛋白的潜力。同时监测蛋白质转运和界面聚合物结构的动态变化是一个实验挑战。我们使用单粒子跟踪（SPT）和荧光相关光谱超分辨率光学波动成像（fcsSOFI）的pH响应性水凝胶的大小和结构的可切换的变化，界面的方法，模型蛋白质，溶菌酶的传输特性。SPT分析揭示了响应于pH变化的水凝胶聚合物中和水凝胶聚合物处的蛋白质转运动力学的可逆切换。fcsSOFI使我们能够将水凝胶和孔的可调异质性与受限扩散和吸附/解吸分布的可逆变化联系起来。我们发现，水凝胶的物理化学异质性决定蛋白质的限制和解吸动力学，特别是在pH条件下，水凝胶溶胀。
Mechanistic details about how local physicochemistry of porous interfaces drives protein transport mechanisms are necessary to optimize biomaterial applications. Cross-linked hydrogels made of stimuli-responsive polymers have potential for active protein capture and release through tunable steric and chemical transformations. Simultaneous monitoring of dynamic changes in both protein transport and interfacial polymer structure is an experimental challenge. We use single-particle tracking (SPT) and fluorescence correlation spectroscopy Super-resolution Optical Fluctuation Imaging (fcsSOFI) to relate the switchable changes in size and structure of a pH-responsive hydrogel to the interfacial met, transport properties of a model protein, lysozyme. SPT analysis reveals the reversible switching of protein transport dynamics in and at the hydrogel polymer in response to pH changes. fcsSOFI allows us to relate tunable heterogeneity of the hydrogels and pores to reversible changes in the distribution of confined diffusion and adsorption/desorption. We find that physicochemical heterogeneity of the hydrogels dictates protein confinement and desorption dynamics, particularly at pH conditions in which the hydrogels are swollen.