Cells preferentially grow on rough substrates
Cells preferentially grow on rough substrates
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DOI：
10.1016/j.biomaterials.2010.06.016
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发表时间：
2010-10-01
期刊：
影响因子：
14
通讯作者：
Decuzzi, Paolo
中科院分区：
文献类型：
--
作者：
Gentile, Francesco;Tirinato, Luca;Decuzzi, Paolo
底物纳米形貌影响细胞的粘附和增殖，是合理设计生物粘合剂、组织工程和开发体外筛选试验的基础。粗糙底物上的细胞行为仍然难以捉摸，公开文献中提出的结果仍然存在争议。在这里，用共聚焦和原子力显微镜研究了细胞在不同粗糙度和几乎相似表面能的电化学蚀刻硅衬底上的增殖。基片表面轮廓为自仿射分形，粗糙度R-a随刻蚀时间的增加而增大，分形维数D在约2（名义平面）至2.6之间。对于四种细胞类型，在中等粗糙（R-a近似于10-45 nm）和接近布朗（D近似于2.5 nm）的底物上，粘附细胞的数量和增殖率最大。观察到的细胞行为在随机粗糙固体的粘附理论中得到了令人满意的解释。这些发现证明了纳米几何结构在细胞稳定粘附和生长中的重要性，表明中等粗糙的大分形维数底物可以选择性地促进细胞增殖。(C) 2010 Elsevier Ltd.版权所有。
Substrate nanotopography affects cell adhesion and proliferation and is fundamental to the rational design of bio-adhesives, to tissue engineering and to the development of assays for in-vitro screening. Cell behavior on rough substrates is still elusive, and the results presented in the open literature remain controversial. Here, the proliferation of cells on electrochemically etched silicon substrates with different roughness and nearly similar surface energy was studied over three days with confocal and atomic force microscopy. The surface profile of the substrates is a self-affine fractal with a roughness R-a growing with the etching time from similar to 2 to 100 nm and a fractal dimension D ranging between about 2 (nominally flat surface) and 2.6. For four cell types, the number of adhering cells and their proliferation rates exhibited a maximum on moderately rough (R-a similar to 10-45 nm) nearly Brownian (D similar to 2.5) substrates. The observed cell behavior was satisfactorily interpreted within the theory of adhesion to randomly rough solids. These findings demonstrated the importance of nanogeometry in cell stable adhesion and growth, suggesting that moderately rough substrates with large fractal dimension could selectively boost cell proliferation. (C) 2010 Elsevier Ltd. All rights reserved.