STUDIES ON THE PRESENCE AND RELEASE OF PROTEOLYTIC-ENZYMES (PROTEINASES) IN GASTROINTESTINAL NEMATODES OF RUMINANTS
STUDIES ON THE PRESENCE AND RELEASE OF PROTEOLYTIC-ENZYMES (PROTEINASES) IN GASTROINTESTINAL NEMATODES OF RUMINANTS
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DOI：
10.1016/0020-7519(90)90106-w
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发表时间：
1990-04-01
影响因子：
4
通讯作者：
JONES, DG
中科院分区：
文献类型：
--
作者：
KNOX, DP;JONES, DG
使用蛋白质底物检查了从绵羊胃肠线虫、线虫、圆线虫、蛇形毛圆线虫、玻璃毛圆线虫和捻转血矛线虫以及啮齿动物肠道线虫巴西尼波圆线虫的第三阶段幼虫（L3）和成虫阶段制备的蠕虫匀浆的蛋白水解活性偶氮酪蛋白、偶氮科尔和弹性蛋白-地黄蛋白。在第三阶段幼虫中检测到的活性通常高于在成虫中观察到的活性。展示了物种和阶段差异。同样，使用蛋白质底物和特定的低分子量肽底物检测到的蛋白水解活性的体外释放也表现出一定程度的物种和阶段特异性。乙酰胆碱酯酶和乳酸脱氢酶活性分别确定为参考“分泌”和“非分泌”酶。对源自 Ostertagiacircumcincta 成虫的培养液进行阴离子交换色谱分析后，检测到三个独立的“胰蛋白酶”活性峰。根据抑制剂敏感性，这些峰可以归因于不同的蛋白酶类别。
The proteolytic activity of worm homogenates prepared from the third stage larval (L3) and adult stages of the ovine gastro-intestinal nematodes, nematodirus battus, Ostertagia circumcincta, Trichostrongylus colubriformis, Trichostrongylus vitrinus and Haemonchus contortus, and the rodent intestinal nematode, Nippostrongylus brasiliensis, has been examined using the protein substrates azocasein, azocoll and elastin-orcein. Activity detected in third stage larvae was usually higher than that observed in the adult. Species and stage differences were demonstrated. The in vitro release of proteolytic activity, detected using protein substrates and specific low molecular weight peptide substrates, was, similarly, shown to exhibit a degree of species and stage specificity. Acetylcholinesterase and lactate dehydrogenase activities were determined as reference ''secretory'' and ''non-secretory'' enzymes, respectively. Three separate peaks of ''tryptic'' activity were detected following anion-exchange chromatography of culture fluids derived from adult Ostertagia circumcincta. These peaks could be ascribed to different proteinase classes on the basis of inhibitor sensitivity.