Distribution of AMP-deaminase isozymes in rat tissues.
Distribution of AMP-deaminase isozymes in rat tissues.
复制标题
大鼠组织中 AMP 脱氨酶同工酶的分布。
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
T. Watanabe
中科院分区：
文献类型：
--
作者：
N. Ogasawara;H. Goto;Y. Yamada;T. Watanabe
1.通过醋酸纤维素膜电泳、磷酸纤维素柱层析以及应用针对三种亲本AMP脱氨酶（同工酶A、B和C）的特异性抗血清的免疫学技术，分析大鼠组织中AMP脱氨酶同工酶的分布。骨骼肌提取物和隔膜提取物含有一种相同的同工酶，即同工酶 A。肝脏、肾脏和睾丸的主要同工酶种类也相同，均为同工酶 B。心脏提取物仅含有同工酶 C。脑、肺和脾的提取物含有五种同工酶，大概是五种 B-C 杂种的完整集合。 2.研究了AMP脱氨酶同工酶的发育规律。在出生后早期，心脏、肝脏、肾脏和肺的提取物含有五种同工酶，与成人大脑中观察到的同工酶相似。在出生后发育过程中，心脏中发生向同工酶 C 的转变，而肝脏和肾脏中发生向同工酶 B 的转变。在整个发育过程中，肺中仍保留着五种同工酶。在大脑中，在发育过程中观察到 B 转变为五种同工酶。同工酶 A 是整个出生后发育过程中肌肉的主要形式。 3.对再生肝脏中的AMP脱氨酶进行了分析，但数据表明，肝脏再生过程中同工酶的分布没有变化。
1. The distribution of AMP deaminase isozymes in rat tissues was analyzed by electrophoresis on cellulose acetate membrane, by chromatography on phosphocellulose column, and by the application of immunological technique employing specific antisera against three parental AMP deaminases (isozymes A, B and C). Skeletal muscle extracts and diaphragm extracts contain a single identical isozyme, isozyme A. The major isozyme species of liver, kidney and testes are also identical and they are isozyme B. Heart extracts contains isozyme C exclusively. Extracts of brain, lung and spleen contain five isozymes, presumably a complete set of five B-C hybrids. 2. Developmental patterns of AMP deaminase isozyme were studied. In early postnatal life, extracts of heart, liver, kidney and lung contain five isozymes similar to those observed in adult brain. During postnatal development, a shift to isozyme C occurs in heart, whereas a shift to isozyme B occurs in liver and kidney. Five isozymes in lung remain throughout development. In brain a shift of B to five isozymes is observed during development. Isozyme A is the predominant form in muscle throughout postnatal development. 3. AMP deaminase in the regenerating liver was analyzed, but the data indicated that there was no change of isozyme distribution during hepatic regeneration.