Odd-paired controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory network
Odd-paired controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory network
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DOI：
10.7554/elife.18215
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发表时间：
2016-08-15
期刊：
影响因子：
7.7
通讯作者：
Akam, Michael
中科院分区：
文献类型：
--
作者：
Clark, Erik;Akam, Michael
果蝇胚胎短暂地表现出双节周期性，由七个“配对规则”基因的表达定义，每个基因以七条条纹的模式表达。在原肠胚形成时，配对规则基因之间的相互作用导致频率加倍和14个parasegment边界的模式化。与果蝇前后模式的早期阶段相反，这种转变还没有得到很好的理解。通过仔细分析的时空动态的配对规则基因的表达，我们表明，频率加倍沉淀的多个协调变化的网络之间的调控相互作用的配对规则基因。我们确定广泛表达，但时间模式的转录因子，奇数配对（Opa/Zic），作为这些变化的原因，并表明偶数parasegment边界的图案依赖于Opa依赖的监管相互作用。我们的研究结果表明，对规则的基因调控网络具有时间调制的拓扑结构，允许对规则的基因发挥阶段特定的模式化作用。
The Drosophila embryo transiently exhibits a double-segment periodicity, defined by the expression of seven 'pair-rule' genes, each in a pattern of seven stripes. At gastrulation, interactions between the pair-rule genes lead to frequency doubling and the patterning of 14 parasegment boundaries. In contrast to earlier stages of Drosophila anteroposterior patterning, this transition is not well understood. By carefully analysing the spatiotemporal dynamics of pair rule gene expression, we demonstrate that frequency-doubling is precipitated by multiple coordinated changes to the network of regulatory interactions between the pair-rule genes. We identify the broadly expressed but temporally patterned transcription factor, Odd-paired (Opa/Zic), as the cause of these changes, and show that the patterning of the even-numbered parasegment boundaries relies on Opa-dependent regulatory interactions. Our findings indicate that the pair-rule gene regulatory network has a temporally modulated topology, permitting the pair-rule genes to play stage-specific patterning roles.