Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes
Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes
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DOI：
10.1016/j.ajog.2007.06.027
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发表时间：
2007-09-01
影响因子：
9.8
通讯作者：
Kim, Chong Jai
中科院分区：
文献类型：
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作者：
Montenegro, Daniel;Romero, Roberto;Kim, Chong Jai
目的：本研究的目的是确定绒毛膜羊膜中微小RNA（miRNA）随着妊娠、分娩和炎症的差异表达。研究设计：从以下组的患者中获得了绒毛膜羊膜中157种miRNA的表达谱：1）足月未分娩（n = 10）; 2）足月分娩（n = 10）;结果：筛选出的miRNAs中95%以上有表达，其中10例为正常对照组，9例为有组织学绒毛膜羊膜炎的早产儿，10例为无组织学绒毛膜羊膜炎的早产儿。在13种miRNAs中观察到了生长年龄依赖性的表达变化。未分娩妇女和分娩妇女的miRNA表达无差异。具有绒毛膜炎的膜显示miR-223和miR-338的表达增加。差异表达的miRNAs靶基因的基因本体论分析显示富集特定的生物过程categorys.CONCLUSION：绒毛膜羊膜与孕龄和绒毛膜炎的进展与一个子集的miRNAs的差异表达。
OBJECTIVE: The aim of this study was to identify differential expression of microRNAs ( miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation.STUDY DESIGN: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10).RESULTS: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories.CONCLUSION: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs.