The human rosette-forming cell as a marker of a population of thymus-derived cells.
The human rosette-forming cell as a marker of a population of thymus-derived cells.
复制标题
人类玫瑰花结形成细胞作为胸腺衍生细胞群的标记。
DOI：
10.1172/jci107069
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
H. Fudenberg
中科院分区：
文献类型：
--
作者：
J. Wybran;M. Carr;H. Fudenberg
在体外，绵羊红细胞可以包围一些人外周血淋巴细胞，形成玫瑰花结。50例正常人玫瑰花结形成细胞（RFC）的数量范围很广（4-40%）。对13例胎龄11-19周的人胎儿的脏器进行RFC检查。胸腺中RFC的百分率最高，在两个15-16周的胎儿标本中最高可达胸腺细胞总数的65%。血液RFC始终存在，其数量在最老的胎儿中略有增加。在研究的6只胎仔中，骨髓显示0-8%。在妊娠13周左右的脾脏和17周左右的肝脏中发现RFC。这些观察结果导致的假设，人类血液RFC可能主要是胸腺衍生的。对免疫系统疾病患者的研究支持这一假设：1例Nezelof综合征患者无血液RFC，4例Wiskott-Aldrich综合征患者血液RFC数量较少（1和1.5%）。获得性低丙种球蛋白血症患者RFC百分比正常。对于胎胸腺细胞，抗mu血清的抑制百分比随胎龄增加而增加，直至在研究的最年长胎中完全抑制。最老的胎儿胸腺细胞或血淋巴细胞与抗γ血清或抗μ血清孵育完全抑制玫瑰花结的形成。这些结果表明，μ链决定簇存在于人胎儿胸腺细胞和血液RFC。这些细胞上存在γ链决定簇的意义尚不清楚。
Sheep red blood cells can surround, in vitro, some human peripheral blood lymphocytes in a formation called a rosette. The number of rosetteforming cells (RFC) in 50 normal persons had a wide range (4-40%). The organs of 13 human fetuses (11-19 wk conceptional age) were examined for the presence of RFC. The thymus possessed the highest percentage of RFC, the maximum being 65% of total thymocytes in two 15-16 wk fetal specimens. Blood RFC were always present and their number slightly increased in the oldest fetuses. The bone-marrow showed 0-8% in the six fetuses studied. RFC were found in the spleen around the 13th wk and in the liver around the 17th wk of gestation. These observations lead to the hypothesis that human blood RFC may be chiefly thymic derived. Studies of patients with immunological disorders support this hypothesis: one patient with Nezelof syndrome had no blood RFC and four patients with Wiskott-Aldrich syndrome had a low number of blood RFC (1 and 1.5%). Patients with acquired hypogammaglobulinemia showed a normal percentage of RFC. With the fetal thymocytes, the percentage of inhibition with anti-mu serum increased with the fetal age to become complete in the oldest fetuses studied. Incubation of the oldest fetal thymocytes or the blood lymphocytes with anti-gamma serum of anti-mu serum completely inhibited the rosette formation. These results suggest that mu-chain determinants are present on human fetal thymocytes and blood RFC. The significance of the presence of gamma-chain determinants on these cells is unclear.