Sticky DNA:: Self-association properties of long GAA•TTC repeats in R•R•Y triplex structures from Friedreich's ataxia
Sticky DNA:: Self-association properties of long GAA•TTC repeats in R•R•Y triplex structures from Friedreich's ataxia
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DOI：
10.1016/s1097-2765(00)80474-8
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发表时间：
1999-04-01
期刊：
影响因子：
16
通讯作者：
Wells, RD
中科院分区：
文献类型：
--
作者：
Sakamoto, N;Chastain, PD;Wells, RD
A novel DNA structure, sticky DNA, is described for lengths of (GAA.TTC)(n) found in intron 1 of the frataxin gene of Friedreich's ataxia patients. Sticky DNA is formed by the association of two purine.purine pyrimidine (R.R.Y) triplexes in negatively supercoiled plasmids at neutral pH. An excellent correlation was found between the lengths of (GAA.TTC) (>59 repeats): first, in FRDA patients, second, required to inhibit transcription in vivo and in vitro, and third, required to adopt the sticky conformation. Fourth, (GAAGGA.TCCTTC)(65), also found in intron 1, does not form sticky DNA, inhibit transcription, or associate with the disease. Hence, R.R.Y triplexes and/or sticky DNA may be involved in the etiology of FRDA.
