Software for the high-throughput collection of SAXS data using an enhanced Blu-Ice/DCS control system.
Software for the high-throughput collection of SAXS data using an enhanced Blu-Ice/DCS control system.
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DOI：
10.1107/s0909049510028566
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发表时间：
2010-11
影响因子：
2.5
通讯作者：
Tainer JA
中科院分区：
文献类型：
--
作者：
Classen S;Rodic I;Holton J;Hura GL;Hammel M;Tainer JA
在斯坦福大学同步辐射实验室的高分子晶体学小组开发的Blu-Ice GUI和分布式控制系统（DCS）已经过优化、扩展和增强，以满足高级光源SIBYLS光束线上SAXS终端站的特定需求。这里报告的定制提供了一个潜在的路线，其他SAXS光束线需要强大和有效的光束线控制软件。生物小角X射线散射（SAXS）通过定义溶液中的形状、构象和组装，为大分子晶体学（MX）提供了强有力的补充数据。尽管SAXS在原则上是结构生物学的最高通量技术，但数据收集在实践中受到当前数据收集软件的限制。在这里，光束线控制软件，历史上开发的MX光束线，在同步加速器SAXS光束线的有效操作和高通量的数据收集的适应报告。在斯坦福大学同步辐射实验室的高分子晶体学小组开发的Blu-Ice GUI和分布式控制系统（DCS）已经过优化、扩展和增强，以满足高级光源SIBYLS光束线上生物SAXS终端站的特定需求。这里报告的定制提供了一个潜在的路线，其他SAXS光束线需要强大和有效的光束线控制软件。由于大量的努力和优化已经进入晶体学软件，晶体学软件的适应和扩展可能被证明是一个通用的策略，以提供先进的SAXS软件的同步加速器社区。通过这种方式，可以将精力投入到优化SAXS的功能中，而不是复制那些已经在晶体学社区成功实现的功能。
The Blu-Ice GUI and Distributed Control System (DCS) developed in the Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory has been optimized, extended and enhanced to suit the specific needs of the SAXS endstation at the SIBYLS beamline at the Advanced Light Source. The customizations reported here provide one potential route for other SAXS beamlines in need of robust and efficient beamline control software. Biological small-angle X-ray scattering (SAXS) provides powerful complementary data for macromolecular crystallography (MX) by defining shape, conformation and assembly in solution. Although SAXS is in principle the highest throughput technique for structural biology, data collection is limited in practice by current data collection software. Here the adaption of beamline control software, historically developed for MX beamlines, for the efficient operation and high-throughput data collection at synchrotron SAXS beamlines is reported. The Blu-Ice GUI and Distributed Control System (DCS) developed in the Macromolecular Crystallography Group at the Stanford Synchrotron Radiation Laboratory has been optimized, extended and enhanced to suit the specific needs of the biological SAXS endstation at the SIBYLS beamline at the Advanced Light Source. The customizations reported here provide a potential route for other SAXS beamlines in need of robust and efficient beamline control software. As a great deal of effort and optimization has gone into crystallographic software, the adaption and extension of crystallographic software may prove to be a general strategy to provide advanced SAXS software for the synchrotron community. In this way effort can be put into optimizing features for SAXS rather than reproducing those that have already been successfully implemented for the crystallographic community.