The enzymatic production of lactulose via transglycosylation in conventional and non-conventional media
The enzymatic production of lactulose via transglycosylation in conventional and non-conventional media
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DOI：
10.1016/j.idairyj.2013.07.010
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Mehrdad, Mahsa
中科院分区：
文献类型：
--
作者：
Khatami, Seyyedhadi;Ashtiani, Farzin Zokaee;Mehrdad, Mahsa
本文研究了乳酸克鲁维酵母（Kluyveromyceslactis）在水相和非传统培养基中利用β-半乳糖苷酶生产乳果糖的情况。采用析因设计方法对水相培养基中乳果糖酶促生产速率进行优化。结果表明，乳糖和果糖水平以及它们的相互作用对乳果糖产生速率有统计学显著影响。当果糖和乳糖浓度分别为25-30%（w/w）和9-12%（w/w）时，获得最大乳果糖生产速率。丙酮的加入对乳果糖生产有负面影响，而掺入浓度高达30%（w/w）的磷酸三乙酯导致乳果糖生产增加高达20%。(C)2013爱思唯尔有限公司保留所有权利。
In this study, lactulose production with beta-galactosidase from Kluyveromyces lactis in aqueous and non-conventional media was studied. The optimisation of the rate of enzymatic lactulose production in aqueous media was carried out using factorial design methodology. The results showed a statistically significant effect of both lactose and fructose levels - as well as their interaction - on the rate of lactulose production. Maximum lactulose production rate was obtained at fructose and lactose concentrations of 25-30% (w/w) and 9-12% (w/w), respectively. The addition of acetone had a negative effect on lactulose production, whereas the incorporation of triethyl phosphate up to a concentration of 30% (w/w) caused an increase of up to 20% in the lactulose production. (C) 2013 Elsevier Ltd. All rights reserved.