BioWardrobe: an integrated platform for analysis of epigenomics and transcriptomics data.
BioWardrobe: an integrated platform for analysis of epigenomics and transcriptomics data.
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DOI：
10.1186/s13059-015-0720-3
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发表时间：
2015-08-07
期刊：
影响因子：
12.3
通讯作者：
Barski A
中科院分区：
文献类型：
--
作者：
Kartashov AV;Barski A
高通量测序通过提高我们进行全基因组研究的能力，使生物学发生了革命性的变化。然而，由于缺乏生物信息学专业知识，现代技术仍然超出了许多实验室的能力。在此，我们介绍了BioWardrobe平台，它允许用户使用一个生物学家友好的网络界面来存储、可视化和分析表观基因组学和转录组数据，而不需要编程专业知识。预定义的管道允许用户下载数据、在基因组浏览器上可视化结果、计算RPKM(每千基每百万个碱基的读数)和识别峰值。高级功能包括差异基因表达和结合分析，以及创建平均标签密度分布和热图。生物衣橱可以在http://biowardrobe.com.上找到本文的在线版本(doi：10.1186/s13059-0150720-3)包含补充材料，授权用户可以使用。
High-throughput sequencing has revolutionized biology by enhancing our ability to perform genome-wide studies. However, due to lack of bioinformatics expertise, modern technologies are still beyond the capabilities of many laboratories. Herein, we present the BioWardrobe platform, which allows users to store, visualize and analyze epigenomics and transcriptomics data using a biologist-friendly web interface, without the need for programming expertise. Predefined pipelines allow users to download data, visualize results on a genome browser, calculate RPKMs (reads per kilobase per million) and identify peaks. Advanced capabilities include differential gene expression and binding analysis, and creation of average tag -density profiles and heatmaps. BioWardrobe can be found at http://biowardrobe.com. The online version of this article (doi:10.1186/s13059-015-0720-3) contains supplementary material, which is available to authorized users.