Acquisition of the Conjugative Virulence Plasmid From a CG23 Hypervirulent Klebsiella pneumoniae Strain Enhances Bacterial Virulence.
Acquisition of the Conjugative Virulence Plasmid From a CG23 Hypervirulent Klebsiella pneumoniae Strain Enhances Bacterial Virulence.
复制标题
从 CG23 高毒力肺炎克雷伯菌菌株中获取接合毒力质粒可增强细菌毒力
DOI：
10.3389/fcimb.2021.752011
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发表时间：
2021
影响因子：
5.7
通讯作者：
Jiang X
中科院分区：
文献类型：
--
作者：
Tian D;Wang W;Li M;Chen W;Zhou Y;Huang Y;Sheng Z;Jiang X
高毒力和耐碳青霉烯类肺炎克雷伯菌 (hv-CRKP) 的出现已成为临床医生和研究人员的热门话题和令人困惑的问题。接合毒力质粒有可能导致 hv-CRKP 菌株更具威胁性的传播。我们之前鉴定了 K2606，这是一种 CG23 临床高毒力肺炎克雷伯菌菌株，含有称为 pK2606 的接合毒力质粒。在这项研究中，我们通过生物膜形成、血清抗性以及蜡幼虫和小鼠体内感染模型的测定来检查高毒力水平。此外，为了确定 pK2606 的转移能力以及这是否赋予其他菌株超毒力，我们在 K2606 和 ST11 CRKP 菌株 HS11286 以及大肠杆菌 J53 之间进行了质粒转接合实验。我们发现，虽然生物膜形成和血清抗性没有显着增加，但转接合子获得了产生高水平铁载体的能力，并且还导致蜡幼虫和小鼠的高死亡率。此外，我们在GenBank中鉴定出了类似pK2606的接合毒力质粒，这提供了此类质粒可能已经开始在中国传播的证据。这些发现为 hv-CRKP 菌株出现的可能机制提供了证据基础，并强调了 pK2606 样接合毒力质粒在全球传播的潜力。
The emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) has become a hot topic and confounding problem for clinicians and researchers alike. Conjugative virulence plasmids have the potential to cause more threatening dissemination of hv-CRKP strains. We previously identified K2606, a CG23 clinical hypervirulent strain of Klebsiella pneumoniae harboring a conjugative virulence plasmid designated pK2606. In this study we examined hypervirulence levels using assays of biofilm formation, serum resistance, and wax larvae and mouse in vivo infection models. Moreover, to define the transfer ability of pK2606 and whether this confers hypervirulence to other strains we performed plasmid transconjugation experiments between K2606 and the ST11 CRKP strain HS11286 along with E. coli J53. We found that although biofilm formation and serum resistance were not significantly increased, the transconjugants acquired the ability of produce high level of siderophores and also caused high mortality of wax larvae and mice. Furthermore, we identified pK2606-like conjugative virulence plasmids in GenBank, providing evidence that such plasmids may have begun to spread throughout China. These findings provide an evidence base for the possible mechanisms of the emergence of hv-CRKP strains and highlight the potential of pK2606-like conjugative virulence plasmids to spread worldwide.