The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter
The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter
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DOI：
10.1073/pnas.120170197
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发表时间：
2000-06-06
影响因子：
11.1
通讯作者：
Zhu, JK
中科院分区：
文献类型：
--
作者：
Shi, HZ;Ishitani, M;Zhu, JK
在拟南芥中，SOS 1（Salt Overly Sensitive 1）位点对于Na+和K+的稳态是必不可少的，并且SOS 1突变使得植物对高Na+和低K+环境的生长抑制更敏感。SOS 1被克隆并预测编码一个127-kDa的蛋白质，在N-末端部分具有12个跨膜结构域，在C-末端部分具有长的亲水性胞质尾。SOS 1的跨膜区与细菌和真菌的质膜Na+/H+反向转运蛋白具有显著的序列相似性。对不同的sos 1突变等位基因的序列分析揭示了跨膜和尾部的几个残基和区域，这些残基和区域对植物耐盐性中的SOS 1功能至关重要。SOS 1基因在NaCl胁迫下表达上调，而在sos 3和sos 2突变体中表达下调，表明其受SOS 3/SOS 2调控途径的调控。
In Arabidopsis thaliana, the SOS1 (Salt Overly Sensitive 1) locus is essential for Na+ and K+ homeostasis, and sos1 mutations render plants more sensitive to growth inhibition by high Na+ and low K+ environments. SOS1 is cloned and predicted to encode a 127-kDa protein with 12 transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail in the C-terminal part. The transmembrane region of SOS1 has significant sequence similarities to plasma membrane Na+/H+ antiporters from bacteria and fungi. Sequence analysis of various sos1 mutant alleles reveals several residues and regions in the transmembrane as well as the tail parts that are critical for SOS1 function in plant salt tolerance. SOS1 gene expression in plants is up-regulated in response to NaCl stress, This up-regulation is abated in sos3 or sos2 mutant plants, suggesting that it is controlled by the SOS3/SOS2 regulatory pathway.