Microfluidic add-on for standard electrophysiology chambers
Microfluidic add-on for standard electrophysiology chambers
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DOI：
10.1039/b802037j
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Eddington, David T.
中科院分区：
文献类型：
--
作者：
Mohammed, Javeed Shaikh;Caicedo, Hector Hugo;Eddington, David T.
我们已经开发出一种微流控脑切片装置（mu BSD），它将现成的脑切片灌注室与设置在室基底的底表面中的微流控通道阵列结合在一起。由于该设备是通过快速原型设计创建的，一旦优化，复制和与其他研究人员共享设备是微不足道的。该设备可无缝集成到标准生理和成像室中，并可立即供整个切片生理学社区使用。有了这项技术，我们可以利用我们选择的时间剖面，将神经化学物质和任何其他可溶性因子的流动定位到大脑切片中的精确位置。多巴胺（DA）被选为模型神经递质，我们已经定量交付在脑组织中使用循环伏安法（CV）和荧光成像。
We have developed a microfluidic brain slice device (mu BSD) that marries an off-the shelf brain slice perfusion chamber with an array of microfluidic channels set into the bottom surface of the chamber substrate. As this device is created through rapid prototyping, once optimized, it is trivial to replicate and share the devices with other investigators. The device integrates seamlessly into standard physiology and imaging chambers and it is immediately available to the whole slice physiology community. With this technology we can address the flow of neurochemicals and any other soluble factors to precise locations in the brain slice with the temporal profile we choose. Dopamine (DA) was chosen as a model neurotransmitter and we have quantified delivery in brain tissue using cyclic voltammetry (CV) and fluorescence imaging.