The distribution and function of human memory T cell subsets in lung cancer
The distribution and function of human memory T cell subsets in lung cancer
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DOI：
10.1007/s12026-016-8882-y
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发表时间：
2017-06-01
影响因子：
4.4
通讯作者：
Wang, RongFu
中科院分区：
文献类型：
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作者：
Sheng, Si Yuan;Gu, Yong;Wang, RongFu
T淋巴细胞在人类肺癌中的分布和功能仍然有限。在这项研究中，我们研究了非小细胞肺癌（NSCLC）患者血液中人T细胞亚群的特性。我们发现，与健康供者相比，非小细胞肺癌患者血液中的CD4+亚群水平相对正常，但CD8+效应T细胞增加，CD8+效应记忆细胞下降。为了进一步分析它们的特性，我们用有丝分裂原刺激非小细胞肺癌患者外周血单个核细胞（PBMCs）来检测细胞因子的产生。我们的数据表明，非小细胞肺癌患者的CD4+和CD8+ na < ve细胞显著降低了ifn - γ和tnf - α的产生。此外，与健康受试者相比，非小细胞肺癌患者的CD8+效应细胞产生的ifn - γ和tnf - α较少。此外，在非小细胞肺癌患者的CD4+或CD8+记忆细胞中，也观察到产生ifn - γ、tnf - α和IL-17的类似结果。因此，我们的研究结果强烈提示，非小细胞肺癌患者的CD4+和CD8+ T淋巴细胞功能受损或失调。疫苗和抗肿瘤免疫疗法的发展可能对肺癌患者的治疗至关重要。
The distribution and function of T lymphocytes in human lung cancer remain limited. In this study, we investigated the properties of human T cell subsets in the blood of non-small cell lung cancer (NSCLC) patients. We found a relatively normal level of CD4+ subsets in the blood of NSCLC patients, but CD8+ effector T cells increased and CD8+ effector memory cells declined compared to the healthy donors. To further analyze their properties, we stimulated the peripheral blood mononuclear cells (PBMCs) of NSCLC patients by mitogens to examine cytokine production. Our data suggest that both CD4+ and CD8+ na < ve cells in NSCLC patients significantly reduced IFN-gamma and TNF-alpha production. Additionally, fewer CD8+ effector cells produced IFN-gamma and TNF-alpha in NSCLC patients than in healthy subjects. Moreover, similar results were observed for CD4+ or CD8+ memory cells in NSCLC patients for the production of IFN-gamma, TNF-alpha, and IL-17. Therefore, our results strongly suggest that the function of CD4+ and CD8+ T lymphocytes in NSCLC patients is compromised or dysregulated. The development of vaccines and antitumor immunotherapy may be essential for the treatment of lung cancer patients.