Effect of pluronic block composition on the structure, stability, and cytotoxicity of liposomes
Effect of pluronic block composition on the structure, stability, and cytotoxicity of liposomes
复制标题
DOI：
10.1080/01932691.2020.1776130
复制
发表时间：
2020-06
影响因子：
2.2
通讯作者：
Zi-ling Li;X. Xiong;Shengfeng Peng;G. Wu;Wei Liu;Cheng-mei Liu
中科院分区：
文献类型：
--
作者：
Zi-ling Li;X. Xiong;Shengfeng Peng;G. Wu;Wei Liu;Cheng-mei Liu
摘要本研究旨在探讨三种普朗尼克（F127，F87和P85）对脂质体结构、稳定性和细胞毒性的影响。采用薄膜蒸发法结合动态高压微射流技术制备了普朗尼克修饰脂质体。经普朗尼克修饰后，脂质体的平均粒径明显减小。傅里叶变换红外光谱和差示扫描量热分析表明，普朗尼克成功地纳入脂质体。Pluronics修饰后脂质体的膜稳定性提高，其顺序为Ls < F87-Ls < F127-Ls < P85-Ls。体外细胞毒性研究表明，F127-Ls和F87-Ls具有良好的生物相容性，P85-Ls在100 ~ 400 mg/L磷脂浓度范围内具有较低的固有毒性。这些结果表明，普朗尼克F127和F87是脂质体修饰的最佳试剂，并且可以潜在地用作药物递送平台。图形摘要
Abstract This study is aimed to investigate the influence of three pluronics (F127, F87, and P85) on the structure, stability, and cytotoxicity of liposomes (Ls). Pluronics-modified liposomes (pluronic-Ls) were prepared by integrating the film evaporation method and dynamic high pressure microfluidization. The average particle size of liposomes decreased significantly after modification with pluronics. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis revealed that pluronics were successfully incorporated into liposomes. The membrane stability of liposomes was improved by pluronics modification, and increased in the following order: Ls < F87-Ls < F127-Ls < P85-Ls. In vitro cytotoxicity studies indicated that F127-Ls and F87-Ls possess good biocompatibility, and P85-Ls exhibited relatively low inherent toxicity in the phospholipid concentrations ranging from 100 to 400 mg/L. These results demonstrated that pluronics F127 and F87 are the optimal agents for liposomes modification and can potentially be used as a drug delivery platform. Graphical Abstract