Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts
Cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate on primary cultured human gingival fibroblasts
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二葡萄糖酸氯己定改良非平衡血浆对原代培养人牙龈成纤维细胞的细胞毒性
DOI：
10.1007/s11596-016-1556-0
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发表时间：
2016-02-01
影响因子：
--
通讯作者：
Cao, Ying-guang
中科院分区：
文献类型：
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作者：
Chen, Hui;Shi, Qi;Cao, Ying-guang
本研究旨在探讨含葡萄糖酸氯己定（CHX）的改良非平衡等离子体对人牙龈成纤维细胞（HGF）的细胞毒性作用，并评价2%CHX改良非平衡等离子体作为根管治疗新方法的生物安全性。对取自人牙龈的组织样本进行原代培养和传代。使用第3-7代的细胞。用含2%CHX的改良非平衡血浆分别处理HGF 0 min（对照组）、30 s、1 min、1.5 min、3 min、5 min和10 min，然后分别孵育0、24和48 h。采用CCK-8法检测含2%CHX的改良非平衡血浆对HGF的细胞毒性。改良非平衡态血浆处理1 min后，0 h组与对照组比较差异无显著性（P > 0.05）。其余各处理组与对照组比较差异均有显著性（P < 0.05）。处理1.5min或更短时间，细胞活力明显增加，处理3 min或更长时间，细胞活力明显降低。此外，当连续培养0，24，和48小时，细胞活力下降，在第一，然后在3分钟处理组和5分钟处理组增加。含2%CHX的改良非平衡等离子体处理1.5min对细胞活力无影响，可安全用于根管治疗。
The aim of this study was to investigate the cytotoxicity of modified nonequilibrium plasma with chlorhexidine digluconate (CHX) on human gingival fibroblasts (HGFs), and to evaluate the biosecurity of modified nonequilibrium plasma with 2% CHX as a new method of root canal treatment. Tissue samples taken from human gingiva were primarily cultured and passaged. Cells from passages 3-7 were used. HGFs were treated by modified nonequilibrium plasma with 2% CHX for 0 min (control group), 30 s, 1 min, 1.5 min, 3 min, 5 min, and 10 min, respectively, and then they were incubated for 0, 24, and 48 h. After that, cell counting kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of modified nonequilibrium plasma with 2% CHX on HGFs. There was no significant difference between the 0 h group treated with the modified nonequilibrium plasma for 1 min and the control group (P > 0.05). However, there were significant differences between all the other treated groups and the control group (P < 0.05). When treated for 1.5 min or shorter, the cell viability was obviously increased; while treated for 3 min or longer, it was obviously reduced. Moreover, when successively cultured for 0, 24, and 48 h, cell viability was decreased at first and then increased in the 3-min-treated and 5-min-treated groups. The modified nonequilibrium plasma with 2% CHX was of no influence on cell viability in 1.5 min treatment, and it could be safely used on root canal treatment.