STUDIES ON HUMAN EPIDERMAL LANGERHANS CELLS .1. ALLO-ACTIVATING AND ANTIGEN-PRESENTING CAPACITY
STUDIES ON HUMAN EPIDERMAL LANGERHANS CELLS .1. ALLO-ACTIVATING AND ANTIGEN-PRESENTING CAPACITY
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DOI：
10.1111/j.1365-3083.1980.tb00006.x
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
THORSBY, E
中科院分区：
文献类型：
--
作者：
BRAATHEN, LR;THORSBY, E
通过吸泡装置将人表皮与真皮分离并用胰蛋白酶解离。通过用兔抗DR抗血清对细胞进行免疫荧光染色判断，获得的表皮细胞悬浮液含有3-5％朗格汉斯细胞。将表皮细胞与纯化的同种异体 T [胸腺来源] 细胞和自体 T 细胞（有或没有结核菌素的 PPD [纯化蛋白衍生物]）共培养。如果存在自体表皮细胞，则可以获得对同种异体表皮细胞的强烈 T 细胞反应，以及对 PPD 的强烈 T 细胞反应。用抗 DR 抗血清和补体预处理表皮细胞消除了这些反应。表皮细胞显然能够替代巨噬细胞的同种异体激活和抗原呈递功能。由于负责的细胞是 DR 阳性的，因此负责这些功能的细胞很可能是朗格汉斯细胞。
Human epidermis was separated from dermis by means of a suction blister device and dissociated with trypsin. The epidermal cell suspensions obtained contained 3-5% Langerhans cells as judged by immunoflurescence staining of the cells with a rabbit anti-DR antiserum. The epidermal cells were co-cultured with purified allogeneic T [thymus-derived] cells and with autologous T cells with or without PPD [purified protein derivative] of tuberculin. A strong T-cell response to allogeneic epidermal cells was obtained, as was a strong T-cell response to PPD, provided autologous epidermal cells were present. Pretreatment of the epidermal cells with anti-DR antiserum plus complement abolished these responses. Epidermal cells are apparently able to substitute for macrophages in the allo-activating and in the antigen-presenting function. Since the responsible cells were DR-positive, it is highly probable that the cells responsible for these functions are the Langerhans cells.