In Vitro Maturation of Viable Islets From Partially Digested Young Pig Pancreas
In Vitro Maturation of Viable Islets From Partially Digested Young Pig Pancreas
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DOI：
10.3727/096368912x662372
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发表时间：
2014-01-01
影响因子：
3.3
通讯作者：
Lakey, Jonathan R. T.
中科院分区：
文献类型：
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作者：
Lamb, Morgan;Laugenour, Kelly;Lakey, Jonathan R. T.
从市场规模的猪中分离胰岛是昂贵的，在分离和组织培养过程中发生了相当大的胰岛碎片损失。胎猪和新生猪产生胰岛素无反应的胰岛样细胞簇，这些细胞簇在长时间后变得对葡萄糖有反应。这两个问题都会影响临床适用性和商业规模。我们已经将我们的努力集中在一个具有成本效益的可扩展的方法分离可行的胰岛素反应性胰岛。年轻的约克郡猪（平均年龄20天，范围4-30天）进行快速胰腺切除术（
Isolation of islets from market-sized pigs is costly, with considerable islet losses from fragmentation occurring during isolation and tissue culture. Fetal and neonatal pigs yield insulin unresponsive islet-like cell clusters that become glucose-responsive after extended periods of time. Both issues impact clinical applicability and commercial scale-up. We have focused our efforts on a cost-effective scalable method of isolating viable insulin-responsive islets. Young Yorkshire pigs (mean age 20 days, range 4-30 days) underwent rapid pancreatectomy (