Intracisternal infusion of magnesium sulfate solution improved reduced cerebral blood flow induced by experimental subarachnoid hemorrhage in the rat
Intracisternal infusion of magnesium sulfate solution improved reduced cerebral blood flow induced by experimental subarachnoid hemorrhage in the rat
复制标题
DOI：
10.1007/s10143-008-0122-z
复制
发表时间：
2008-02
影响因子：
2.8
通讯作者：
K. Mori;M. Miyazaki;J. Iwata;Takuji Yamamoto;Y. Nakao
中科院分区：
文献类型：
--
作者：
K. Mori;M. Miyazaki;J. Iwata;Takuji Yamamoto;Y. Nakao
镁在蛛网膜下腔出血（SAH）时具有神经保护和抗血管痉挛特性。本研究探讨了脑池内注射镁对实验性SAH大鼠模型脑血管痉挛的影响。使用大鼠双SAH模型（0.2 mL自体血两次注射到枕大池中）。SAH组（N= 8）和SAH + MG组（N = 8）分别于第5天以1.5 μL/min的速度向枕大池注入生理盐水或10 mmol/L硫酸镁生理盐水溶液30 min。无SAH的对照大鼠也接受脑池内输注生理盐水（对照组，N= 6）。局部脑血流量（CBF）在24个位置和加权平均值进行了定量测量的放射自显影技术，使用[14 C]碘安替比林在输液。与对照组（1.0 ± 0.15 mL g− 1 min −1）相比，SAH组（0.78 ± 0.16 mL g− 1 min − 1）的加权平均CBF显著降低（P <0.01，Student检验），SAH + MG组（0.98 ± 0.18 mL g− 1 min −1）的加权平均CBF显著改善（P<0.01，Student检验）。SAH组16个部位局部CBF明显降低（P< 0.05，未配对t检验），SAH + MG组12个部位局部CBF明显改善（P< 0.05，未配对t检验）。脑池内注射硫酸镁可明显改善SAH引起的脑血流量减少。
Magnesium has neuroprotective and antivasospastic properties in the presence of subarachnoid hemorrhage (SAH). The present study investigated the effect of intracisternal administration of magnesium on cerebral vasospasm in the experimental SAH rat model. The rat double-SAH model (0.2 mL autologous blood injected twice into the cisterna magna) was used. Normal saline (SAH group,N= 8) or 10 mmol/L magnesium sulfate in normal saline (SAH + MG group,N= 8) was infused into the cisterna magna at 1.5 μL/min for 30 min on day 5. Control rats without SAH also received intracisternal infusion of normal saline (control group,N= 6). Local cerebral blood flow (CBF) at 24 locations and the weighted average were quantitatively measured by the autoradiographic technique using [14C]iodoantipyrine during infusion. The weighted average CBF was significantly reduced (P< 0.01, Student’st-test) in the SAH group (0.78 ± 0.16 mL g−1min−1) compared to the control group (1.0 ± 0.15 mL g−1min−1) and was significantly improved (P< 0.01, Student’st-test) in the SAH + MG group (0.98 ± 0.18 mL g−1min−1). Local CBF was significantly reduced (P< 0.05, unpairedttest) in 16 locations in the SAH group and significantly improved (P< 0.05, unpairedttest) in 12 locations in the SAH + MG group. Intracisternal infusion of magnesium sulfate significantly improved reduced CBF induced by experimental SAH in the rat.