Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer Pinus densiflora
Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer Pinus densiflora
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DOI：
10.1007/s10265-010-0384-8
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Funada, Ryo
中科院分区：
文献类型：
--
作者：
Nakaba, Satoshi;Kubo, Takafumi;Funada, Ryo
程序性细胞死亡的一个关键事件是核DNA片段化。利用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）研究了赤松短寿命射线管胞细胞死亡过程中细胞核DNA断裂的时间。荧光由于TUNEL检测到只有在变形的细胞核，缺乏明显的染色质射线管胞，射线管胞相邻的射线管胞，不再包含细胞核。我们的观察结果表明，核DNA片段化只发生在射线管胞原位细胞死亡的最后阶段。
One key event in the programmed cell death is nuclear DNA fragmentation. We investigated the timing of nuclear DNA fragmentation during the cell death of short-lived ray tracheids in Pinus densiflora using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fluorescence due to TUNEL was detected only in deformed nuclei that lacked obvious chromatin in ray tracheids that were adjacent to ray tracheids that no longer contained nuclei. Our observations revealed that nuclear DNA fragmentation occurred only at the final stage of cell death in ray tracheids in situ.