Phenotypic effects of the U-genome variation in nascent synthetic hexaploids derived from interspecific crosses between durum wheat and its diploid relative Aegilops umbellulata
Phenotypic effects of the U-genome variation in nascent synthetic hexaploids derived from interspecific crosses between durum wheat and its diploid relative Aegilops umbellulata
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DOI：
10.1371/journal.pone.0231129
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发表时间：
2020-04
期刊：
影响因子：
3.7
通讯作者：
Moeko Okada;Asami Michikawa;Kentaroh Yoshida;K. Nagaki;T. Ikeda;S. Takumi
中科院分区：
文献类型：
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作者：
Moeko Okada;Asami Michikawa;Kentaroh Yoshida;K. Nagaki;T. Ikeda;S. Takumi
小叶盾是一种具有UU基因组的野生二倍体小麦品种，是小麦育种的重要遗传资源。为开发可作为小麦育种桥梁的合成异源六倍体新品系，通过硬粒小麦品种兰登与26个品种的杂交，获得了26个合成六倍体品系。umbellulata。在具有AABBUU基因组的新生合成六倍体中，使用基于Ae的rna -seq数据开发的u -基因组染色体特异性标记证实了7组u -基因组染色体的存在。umbellulata。AABBUU合成的六倍体在开花和形态相关性状上表现出较大的变异，这些变异很好地遗传自亲本Ae。umbellulata登记入册。然而，在AABBUU六倍体背景下，大多数性状的变异范围比在二倍体背景下减小。AABBUU和AABBDD合成的六倍体具有明显的形态特征，AABBUU合成的六倍体株高增加，穗数增加，穗长减少。因此，种间差异的几种形态特征。伞形植物和牛头草对合成六倍体的基本植物结构影响较大。综上所述，本研究获得的AABBUU合成六倍体系为Ae所需基因的导入提供了有用的资源。伞状花序到普通小麦。
Aegilops umbellulata is a wild diploid wheat species with the UU genome that is an important genetic resource for wheat breeding. To exploit new synthetic allohexaploid lines available as bridges for wheat breeding, a total of 26 synthetic hexaploid lines were generated through crossing between the durum wheat cultivar Langdon and 26 accessions of Ae. umbellulata. In nascent synthetic hexaploids with the AABBUU genome, the presence of the set of seven U-genome chromosomes was confirmed with U-genome chromosome-specific markers developed based on RNA-seq-derived data from Ae. umbellulata. The AABBUU synthetic hexaploids showed large variations in flowering- and morphology-related traits, and these large variations transmitted well from the parental Ae. umbellulata accessions. However, the variation ranges in most traits examined were reduced under the AABBUU hexaploid background compared with under the diploid parents. The AABBUU and AABBDD synthetic hexaploids were clearly discriminated by several morphological traits, and an increase of plant height and in the number of spikes and a decrease of spike length were commonly observed in the AABBUU synthetics. Thus, interspecific differences in several morphological traits between Ae. umbellulata and A. tauschii largely affected the basic plant architecture of the synthetic hexaploids. In conclusion, the AABBUU synthetic hexaploid lines produced in the present study are useful resources for the introgression of desirable genes from Ae. umbellulata to common wheat.