Transglutaminase regulates immune-related genes in shrimp
Transglutaminase regulates immune-related genes in shrimp
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DOI：
10.1016/j.fsi.2012.01.018
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发表时间：
2012-05-01
影响因子：
4.7
通讯作者：
Hirono, Ikuo
中科院分区：
文献类型：
--
作者：
Fagutao, Fernand E.;Maningas, Mary Beth B.;Hirono, Ikuo
转谷氨酰胺酶(TGase)参与凝血，这是无脊椎动物的一种保守的防御机制。先前研究表明，TGase的基因沉默使对虾对细菌和病毒感染都很敏感，这表明TGase是对虾免疫系统的重要组成部分。在这里，我们通过微阵列分析来检测缺失TGase对库鲁玛对虾转录图谱的影响，重点关注与对虾免疫相关的基因。分别于注射TGase特异性dsRNA的对虾血细胞和对照样品中提取总RNA。并用基因芯片进行分析。结果表明，TGase沉默影响了对虾基因的表达，并导致粗面蛋白和溶菌酶的表达显著下调。此外，TGase耗尽的样本发现其血淋巴中的血细胞计数较低，而细菌总数较高。这些结果表明，TGase是对虾免疫反应的重要组成部分，参与了一些免疫相关基因的调控，特别是抗菌肽的调控。(C)2012爱思唯尔有限公司。保留所有权利。
Transglutaminase (TGase) is known to be involved in blood coagulation, a conserved defence mechanism among invertebrates. Gene silencing of TGase was previously shown to render shrimp susceptible to both bacterial and viral infections suggesting that TGase is an essential component of the shrimp immune system. Here, we examine the effects of the absence of TGase on the transcriptomic profile of kuruma shrimp by microarray analysis, focussing on genes that are involved in shrimp immunity. Total RNAs from shrimp haemocytes injected with dsRNA specific for TGase and control samples were isolated at 3 and 7 days p.i. and analyzed by microarray. Results revealed that TGase silencing affects the expression of genes in shrimp and caused significant down-regulation of the expressions of crustin and lysozyme. Furthermore, TGase-depleted samples were found to have lower haemocyte counts and higher total bacterial counts in their haemolymph. These results suggest that TGase is an important component of the shrimp immune response and is involved in the regulation of some immune-related genes particularly antimicrobial peptides. (C) 2012 Elsevier Ltd. All rights reserved.