Non-thermal plasma elicits ferrous chloride-catalyzed DMPO-OH
Non-thermal plasma elicits ferrous chloride-catalyzed DMPO-OH
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DOI：
10.1080/10715762.2022.2157272
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发表时间：
2022-12-13
影响因子：
3.3
通讯作者：
Toyokuni，Shinya
中科院分区：
文献类型：
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作者：
Okazaki，Yasumasa;Ito，Nanami;Toyokuni，Shinya
非热等离子体（NTP）在接近生理温度下诱导活性氧（ROS）和活性氮（如羟基自由基（·OH）、过氧化氢（H2 O2）、单线态氧、超氧化物、臭氧和一氧化氮）的产生。这些分子促进血液凝固、伤口愈合、消毒和选择性癌细胞死亡。基于这些证据，已经进行了NTP用于治疗慢性伤口和头颈癌的临床试验。虽然临床应用取得了进展，NTP诱导的ROS的化学计量定量仍然不清楚在液相中的FeCl 2或FeCl 3与生物相容性还原剂，这可能会调节NTP的最终生物效应的组合存在。在这项研究中，我们采用电子顺磁共振光谱定量ROS使用自旋捕获探针，5，5-二甲基-1-吡咯啉-N-氧化物（DMPO）和H2 O2，使用发光探针在FeCl 2或FeCl 3的存在下。NTP诱导的DMPO-OH水平升高10-100 µM FeCl 2或500和1000 µM FeCl 3。抗坏血酸、α-生育酚、二硫苏糖醇、还原型谷胱甘肽或氧化型谷胱甘肽可显著清除10 µM FeCl 2或FeCl 3诱导的DMPO-OH，而脱氢抗坏血酸在2 mM DMPO中无效。100 µM FeCl 2和FeCl 3以铁依赖性方式显著降解NTP诱导的H2 O2。同时，在铁的存在下，过氧化氢酶分解H2 O2能有效降解DMPO-OH，表明铁同时导致DMPO-OH的产生和降解.这些结果表明，NTP诱导的DMPO-OH产生的H2 O2消耗，铁依赖的芬顿反应和铁的中间体。还讨论了潜在的铁介导的活性氧产生的NTP，以澄清NTP诱导的活性氧和生物分子之间的相互作用。
Non-thermal plasma (NTP) induces the generation of reactive oxygen species (ROS) and reactive nitrogen species, such as hydroxyl radicals (•OH), hydrogen peroxide (H2O2), singlet oxygen, superoxide, ozone, and nitric oxide, at near-physiological temperatures. These molecules promote blood coagulation, wound healing, disinfection, and selective cancer cell death. Based on these evidences, clinical trials of NTP have been conducted for treating chronic wounds and head and neck cancers. Although clinical applications have progressed, the stoichiometric quantification of NTP-induced ROS remains unclear in the liquid phase in the presence of FeCl2or FeCl3in combination with biocompatible reducing agents, which may modulate the final biological effects of NTP. In this study, we employed electron paramagnetic resonance spectroscopy to quantify ROS using spin-trapping probe, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and H2O2, using luminescent probe in the presence of FeCl2or FeCl3. NTP-induced DMPO-OH levels were elevated 10–100 µM FeCl2or 500 and 1000 µM FeCl3. NTP-induced DMPO-OH with 10 µM FeCl2or FeCl3was significantly scavenged by ascorbate, α-tocopherol, dithiothreitol, reduced glutathione, or oxidized glutathione, whereas dehydroascorbate was ineffective in 2 mM DMPO. NTP-induced H2O2was significantly degraded by 100 µM FeCl2and FeCl3in an iron-dependent manner. Meanwhile, decomposition of H2O2by catalase decayed DMPO-OH efficiently in the presence of iron, indicating iron causes DMPO-OH production and degradation simultaneously. These results suggest that NTP-induced DMPO-OH is generated by the H2O2-consuming, iron-dependent Fenton reaction and ferryl intermediates. The potential iron-mediated ROS production by NTP is also discussed to clarify the interaction between NTP-induced ROS and biomolecules.