Application of next-generation sequencing to the study of non-model insects
Application of next-generation sequencing to the study of non-model insects
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二代测序在非模式昆虫研究中的应用
DOI：
10.1111/ens.12281
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发表时间：
2018
影响因子：
0.9
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Wachi;N.;Matsubayashi;K.W.;Maeto;K.
平台、技术以及计算机软件和硬件的最新发展和广泛应用增强了遗传标记的使用，以解决非模型生物中的重大科学问题，即使对于基因组学专业知识有限的研究人员也是如此。然而，很少有研究使用这些方法研究非模型昆虫的基因组。这篇综述讨论了下一代测序（NGS）技术在野生生物基因组研究中的应用。我们首先介绍目前可用的基于 NGS 的方法，包括限制性位点相关的 DNA 测序；通过测序进行多重简单序列重复基因分型；目标捕获；以及扩增子、转录组和全基因组测序——作为研究非模式昆虫的有用工具。我们还为 NGS 系统的首次用户提供指南。除了提供大量信息之外，NGS 数据的一个主要优势是未来研究项目的可扩展性。使用NGS技术的研究可以通过以模式生物不可能的方式关注野生环境中非模式昆虫的独特性质来回答与基础昆虫学相关的问题。
Recent developments in, and widespread availability of, platforms, technologies, and computer software and hardware have enhanced the use of genetic markers to address major scientific questions in non‐model organisms, even by researchers with limited expertise in genomics. However, there are few studies investigating the genomes of non‐model insects using these approaches. This review discusses the application of next‐generation sequencing (NGS) technologies to the study of genomes of wild organisms. We first introduce currently available NGS‐based methods—including restriction site‐associated DNA sequencing; multiplexed inter‐simple sequence repeat genotyping by sequencing; target capture; and amplicon, transcriptome, and whole genome sequencing—as useful tools for studies of non‐model insects. We also provide guidelines for first‐time users of NGS systems. In addition to the massive amount of information that it provides, a major advantage of NGS data is the scalability to future research projects. Studies using NGS technology can answer questions related to basic entomology by focusing on the unique nature of non‐model insects in wild environments in a way that is not possible for model organisms.