Naltrexone induces arcuate nucleus neuropeptide Y gene expression in the rat
Naltrexone induces arcuate nucleus neuropeptide Y gene expression in the rat
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DOI：
10.1152/ajpregu.1996.271.1.r289
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发表时间：
1996-07-01
影响因子：
2.8
通讯作者：
Levine, AS
中科院分区：
文献类型：
--
作者：
Kotz, CM;Grace, MK;Levine, AS
神经肽Y（NPY）对能量代谢的几个组成部分具有强效作用，包括增加摄食和减少棕色脂肪产热。能量负平衡时，如食物剥夺，下丘脑弓状核（ARC）内NPY mRNA表达增加。阿片受体拮抗剂纳洛酮（Naltenone，NLTX）可减少NPY诱导的摄食。我们假设NLTX会改变ARC NPY mRNA并改变NPY对棕色脂肪的影响。在32只雄性Sprague-Dawley大鼠皮下植入预先填充有盐水或NLTX（70 μ g/h）的渗透微型泵。一半大鼠禁食，另一半大鼠自由进食48 h。在24和48 h测量摄食量。在48小时，ARC神经肽Y mRNA和棕色脂肪解偶联蛋白（UCP）的mRNA水平测定使用cDNA探针。NLTX输注后48小时的摄食量显著减少24%。食物剥夺和NLTX治疗显着和独立的增加ARC NPY mRNA和减少UCP mRNA水平在棕色脂肪，表明下丘脑NPY和内源性阿片类物质之间的复杂的相互作用，在调节能量平衡。
Neuropeptide Y (NPY) has potent effects on several components of energy metabolism, including increased feeding and decreased brown fat thermogenesis. Negative energy balance, such as food deprivation, increases NPY mRNA in hypothalamic arcuate nucleus (ARC). Naltrexone (NLTX), an opioid receptor antagonist, decreases NPY-induced feeding. We hypothesized that NLTX would alter ARC NPY mRNA and change NPY effects on brown fat. Osmotic minipumps prefilled with either saline or NLTX (70 mu g/h) were implanted subcutaneously in 32 male Sprague-Dawley rats. One-half of the rats were food deprived and one-half were allowed food ad libitum for 48 h. Food intake was measured at 24 and 48 h. At 48 h, ARC NPY mRNA and brown fat uncoupling protein (UCP) mRNA levels were determined using cDNA probes. Forty-eight-hour food intake was significantly decreased by 24% after NLTX infusion. Food deprivation and NLTX treatment significantly and independently increased ARC NPY mRNA and decreased UCP mRNA levels in brown fat, suggesting a complex interaction between hypothalamic NPY and endogenous opioids in the regulation of energy balance.