The mouse CD7 gene: identification of a new element common to the human CD7 and mouse Thy-1 promoters
The mouse CD7 gene: identification of a new element common to the human CD7 and mouse Thy-1 promoters
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小鼠 CD7 基因：人类 CD7 和小鼠 Thy-1 启动子共有的新元件的鉴定
作者：
David M. Lee;L. Schanberg;D. Fleenor;M. Seldin;B. Haynes;R. Kaufman
人CD 7（human CD 7，hCD 7）是免疫球蛋白基因超家族中的一个40 000 Mr的成员，在自然杀伤细胞（NK）和T淋巴细胞发育的早期表达。 CD 7参与淋巴细胞活化，因为CD 7的连接活化NK和TCRγδ T淋巴细胞，并且TCRαβ T淋巴细胞上的CD 7的连接诱导非促有丝分裂钙流。我们之前已经克隆并表征了人CD 7（hCD 7）的基因，并描述了其在转基因小鼠中的表达。最近报道了与hCD 7同源的小鼠cDNA，我们将其定位到相应的小鼠染色体位置作为hCD 7。我们现在报告小鼠CD 7（mCD 7）基因组克隆的鉴定和表征。我们证明了mCD 7基因在大小和结构组织上与hCD 7相似。比较mCD 7和hCD 7基因的5′侧翼序列，发现两个区域的序列相似性。电泳迁移率变动分析证实，这两个区域是组织限制性蛋白结合的网站在体外。3′端相似性较高的区域也与小鼠Thy-1基因5′端侧翼区的一个区域共享序列，表明该序列可能是所有三个基因共有的顺式作用调控元件。因此，启动子区域和外显子组织在人CD 7、小鼠CD 7和小鼠Thy-1基因中相似。
Abstract Human CD7 (hCD7) is a 40 000 Mr member of the immunoglobulin gene superfamily that is expressed early in natural killer (NK) and T-lymphocyte development. CD7 is involved in lymphocyte activation, as ligation of CD7 activates NK and TCRγδ T lymphocytes, and ligation of CD7 on TCRαβ T lymphocytes induces a non-mitogenic calcium flux. We have previously cloned and characterized the gene for human CD7 (hCD7) and have described its expression in transgenic mice. Recently a mouse cDNA homologous to hCD7 was reported, which we mapped to the corresponding mouse chromosomal location as hCD7. We now report the identification and characterization of a mouse CD7 (mCD7) genomic clone. We demonstrated that the mCD7 gene was similar both in size and structural organization to hCD7. Comparison of the 5′ flanking sequences of the mCD7 and hCD7 genes revealed two regions of sequence similarity. Electrophoretic mobility shift assay confirmed both of these regions to be sites of tissue-restricted protein binding in vitro. The more 3′ similarity region also shared sequence with a region in the mouse Thy-1 gene 5′ flanking region, suggesting that this sequence may be a cis-acting regulatory element common to all three genes. Thus, the promoter regions and exonic organization were similar in the human CD7, mouse CD7, and mouse Thy-1 genes.
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核因子与β-珠蛋白基因启动子的近端和远端CACCC基序的结合：对-101（C-->T）“沉默”β-地中海贫血突变的影响。
影响因子：
2.4
作者：
Baysal,E;Ribeiro,ML;Huisman,TH
通讯作者：
Huisman,TH
影响因子：
4.3
作者：
JUNG, LKL;ROY, AK;CHAKKALATH, HR
通讯作者：
CHAKKALATH, HR
DOI：
10.1073/pnas.81.1.253
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HERSHFIELD, MS;KURTZBERG, J;HAYNES, BF
通讯作者：
HAYNES, BF
人胎儿骨髓中共表达 CD7 的新型 B 谱系细胞的鉴定。
影响因子：
20.3
作者：
Grümayer,ER;Griesinger,F;Hummell,DS;Brunning,RD;Kersey,JH
通讯作者：
Kersey,JH
非有丝分裂离子霉素诱导的跨膜钙流诱导 T 细胞 CD7 基因转录。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ware,RE;Hart,MK;Haynes,BF
通讯作者：
Haynes,BF