2-Aminophenol and 4-aminophenol toxicity in renal slices from Sprague-Dawley and Fischer 344 rats.
2-Aminophenol and 4-aminophenol toxicity in renal slices from Sprague-Dawley and Fischer 344 rats.
复制标题
Sprague-Dawley 和 Fischer 344 大鼠肾切片中 2-氨基苯酚和 4-氨基苯酚的毒性。
DOI：
10.1080/009841098158511
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ball,JG
中科院分区：
文献类型：
--
作者：
Valentovic,MA;Ball,JG
这项研究使用肾皮质切片模型检验了2-氨基苯酚和4-氨基苯酚的毒性差异。2-和4-氨基苯酚的肾皮质切片毒性也在SpragueDawley和Fischer 344(F344)大鼠的组织中进行了监测，以确定氨基苯酚毒性的潜在品系差异。取SD大鼠和F344大鼠(50~65d)肾皮质切片，在37℃氧气气氛下与0~1mM2或4-氨基苯酚孵育15~120min。两种品系大鼠肾皮质切片乳酸脱氢酶(LDH)漏出量在4-氨基苯酚浓度低于2-氨基苯酚浓度时升高。与2-氨基酚相比，4-氨基酚对两株猪肾切片中总谷胱甘肽水平的降低更为明显。1mM2-氨基苯酚可使F344大鼠肾切片LDH释放量增加，而对照组和治疗组大鼠肾切片LDH释放量无明显差异。4-氨基苯酚对F344大鼠肾切片的毒性稍强于SD大鼠。与对照组相比，F344组大鼠的LDH释放增加了0.1mM，而SpragueDawley组增加了0.25mM。当比较总谷胱甘肽水平或糖异生的限速底物时，菌株的差异并不明显。这些结果表明，SpragueDawley和F344大鼠品系的毒性存在品系差异。根据LDH的释放，年龄匹配的F344大鼠的肾皮质切片比SpragueDawley大鼠对2-和4-氨基苯酚的毒性略敏感。
This study examined differences in toxicity between 2- and 4-aminophenol using a renal cortical slice model. Renal cortical slice toxicity for 2- and 4-aminophenol was also monitored in tissue from Sprague-Dawley and Fischer 344 (F344) rats in order to determine potential strain differences for aminophenol toxicity. Renal cortical slices from Sprague-Dawley and F344 rats (age 50-65 d) were isolated and incubated for 15-120 min with 0-1 m M 2- or 4-aminophenol at 37 C under an oxygen atmosphere. Elevations in lactate dehydrogenase (LDH) leakage from renal cortical slices occurred at lower concentrations of 4-aminophenol than of 2-aminophenol from both strains of rats. Total glutathione levels were more markedly decreased by 4-aminophenol than by 2-aminophenol in renal slices from both strains. LDH release was elevated by 1 m M 2-aminophenol in renal slices from F344 rats, but values were comparable between control and treated in the renal slices from Sprague-Dawley rats. 4-Aminophenol was slightly more toxic to renal slices from F344 than from Sprague-Dawley rats. LDH release was increased, relative to controls, by 0.1 m M in the F344 rats group compared to 0.25 m M in the Sprague-Dawley group. Strain differences were not apparent when comparisons were made of total glutathione levels or rate-limiting substrates of gluconeogenesis. These results indicated that strain differences in toxicity were detected between SpragueDawley and F344 rat strains. Based on LDH release, renal cortical slices obtained from age-matched F344 rats were slightly more susceptible than Sprague-Dawley rats to toxicity by 2- and 4-aminophenol.