Quantifying collagen fiber orientation in breast cancer using quantitative phase imaging
Quantifying collagen fiber orientation in breast cancer using quantitative phase imaging
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DOI：
10.1117/1.jbo.22.4.046004
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发表时间：
2017-04-01
影响因子：
3.5
通讯作者：
Popescu, Gabriel
中科院分区：
文献类型：
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作者：
Majeed, Hassaan;Okoro, Chukwuemeka;Popescu, Gabriel
乳腺癌中的肿瘤进展受到其与周围基质组织的相互作用的显著影响。具体而言，肿瘤相邻基质中胶原纤维的组成、取向和排列影响肿瘤生长和转移。测量这种预后标志物的大部分工作涉及使用二次谐波发生显微镜（SHGM）对胶原纤维进行成像，这提供了无标记的特异性。在这里，我们表明，空间光干涉显微镜（SLIM），一种无标记的定量相位成像技术，能够提供胶原纤维取向的信息，这是由SHGM提供的。由于其宽场几何形状，SLIM系统的吞吐量远远高于SHGM，并且由于线性成像，设备更简单且成本更低。我们的研究结果表明，SLIM图像可用于提取重要的预后信息，从乳腺组织中的胶原纤维，潜在地提供了一个方便的高通量的临床工具，用于评估患者的预后。(C)2017年，摄影光学仪器工程师协会（SPIE）
Tumor progression in breast cancer is significantly influenced by its interaction with the surrounding stromal tissue. Specifically, the composition, orientation, and alignment of collagen fibers in tumor-adjacent stroma affect tumor growth and metastasis. Most of the work done on measuring this prognostic marker has involved imaging of collagen fibers using second-harmonic generation microscopy (SHGM), which provides label-free specificity. Here, we show that spatial light interference microscopy (SLIM), a label-free quantitative phase imaging technique, is able to provide information on collagen-fiber orientation that is comparable to that provided by SHGM. Due to its wide-field geometry, the throughput of the SLIM system is much higher than that of SHGM and, because of the linear imaging, the equipment is simpler and significantly less expensive. Our results indicate that SLIM images can be used to extract important prognostic information from collagen fibers in breast tissue, potentially providing a convenient high throughput clinical tool for assessing patient prognosis. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)