Trophic action of local intraileal infusion of insulin-like growth factor I: polyamine dependence.
Trophic action of local intraileal infusion of insulin-like growth factor I: polyamine dependence.
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局部回肠内输注胰岛素样生长因子 I 的营养作用：多胺依赖性。
DOI：
10.1152/ajpendo.1992.263.2.e282
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Seidel,ER
中科院分区：
文献类型：
--
作者：
Olanrewaju,H;Patel,L;Seidel,ER
进行实验以确定人重组胰岛素样生长因子I（hrIGF-I）在胃肠道中的潜在促生长作用。IGF-I和IGF-II，但不是胰岛素，是肠源性细胞系IEC-6中生长相关酶鸟氨酸脱羧酶（ODC）的强效（半最大有效浓度0.3 nM）和有效诱导剂。用10 nM IGF-I处理细胞后观察到最大ODC诱导。在整个动物实验中，在麻醉大鼠中回肠内推注10 nM hrIGF-I诱导回肠粘膜ODC活性增加300%，其对二氟甲基鸟氨酸（DFMO）的抑制敏感。向大鼠腹膜内植入充满0.9%NaCl或10 nM IGF-I的渗透微型泵，通过短Silastic导管将其递送至回肠腔。与输注NaCl的大鼠相比，1 μ L/h的IGF-I管腔内输注66小时产生的粘膜湿重（NaCl 50 mg vs. IGF-I 102 μ g/2 cm粘膜）和总粘膜RNA、DNA和蛋白质含量约加倍。200 mg/kg DFMO每日3次腹腔给药对回肠粘膜质量影响不大，但完全抑制了IGF-I输注的营养反应。IGF-I输注对体重无影响。
Experiments were performed to determine potential growth-promoting effects of human recombinant insulin-like growth factor I (hrIGF-I) in the gastrointestinal tract. IGF-I and IGF-II, but not insulin, were potent (half-maximal effective concentration 0.3 nM) and efficacious inducers of the growth-related enzyme ornithine decarboxylase (ODC) in the gut-derived cell line IEC-6. Maximal ODC induction was observed after treatment of cells with 10 nM IGF-I. In whole animal experiments, bolus intraileal injection of 10 nM hrIGF-I in anesthetized rats induced a 300% increase in ileal mucosal ODC activity, which was sensitive to inhibition with difluoromethylornithine (DFMO). Rats were implanted intraperitoneally with osmotic minipumps filled with 0.9% NaCl or 10 nM IGF-I that was delivered to the ileal lumen by a short Silastic catheter. Sixty-six hours of 1 microliter/h intraluminal IGF-I infusion produced an approximate doubling of mucosal wet weight (NaCl 50 mg vs. IGF-I 102 micrograms/2 cm mucosa) and total mucosal RNA, DNA, and protein content over that in rats that were infused with NaCl. Intraperitoneal treatment with 200 mg/kg DFMO three times per day had little effect on ileal mucosal mass, but completely inhibited the trophic response to IGF-I infusion. IGF-I infusion had no effect on body weight.