Use of gHgL for attachment of Epstein-Barr virus to epithelial cells compromises infection
Use of gHgL for attachment of Epstein-Barr virus to epithelial cells compromises infection
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DOI：
10.1128/jvi.78.10.5007-5014.2004
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Hutt-Fletcher, LM
中科院分区：
文献类型：
--
作者：
Borza, CM;Morgan, AJ;Hutt-Fletcher, LM
eb病毒（EBV）是一种嗜淋巴性疱疹病毒。然而，在原发性感染期间，粘膜上皮细胞的复制可能促进B淋巴细胞的进入。EBV对这两种细胞类型的附着和渗透是根本不同的。受体的分布和病毒的细胞起源都影响感染的效率。上皮细胞可能提供多种与病毒相互作用的受体。我们在此报告对表达不同受体组合的上皮细胞的分析。我们发现，包括gHgL和gp42在内的病毒糖蛋白复合物的化学计量不仅影响gHgL进入上皮细胞，而且影响其附着。gp42或gHgL的辅助受体都可以有效地介导渗透，但使用gHgL进行附着和渗透极大地损害了其介导进入的能力。
Epstein-Barr virus (EBV) is a lymphotropic herpesvirus. However, access to B lymphocytes during primary infection may be facilitated by replication in mucosal epithelial cells. Attachment and penetration of EBV into these two cell types are fundamentally different. Both the distribution of receptors and the cellular origin of the virus impact the efficiency of infection. Epithelial cells potentially offer a wide range of receptors with which virus can interact. We report here on analyses of epithelial cells expressing different combinations of receptors. We find that the stoichiometry of the virus glycoprotein complex that includes gHgL and gp42 affects the use of gHgL not just for entry into epithelial cells but also for attachment. Penetration can be mediated efficiently with either a coreceptor for gp42 or gHgL, but the use of gHgL for attachment as well as penetration greatly compromises its ability to mediate entry.