[Sharp changes in the copy number of mtDNA and its transcription in the blood cells of X-ray irradiated mice are observed, and mtDNA fragments appear in the blood serum].
[Sharp changes in the copy number of mtDNA and its transcription in the blood cells of X-ray irradiated mice are observed, and mtDNA fragments appear in the blood serum].
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X射线照射小鼠血细胞中mtDNA拷贝数及其转录发生急剧变化，血清中出现mtDNA片段。
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
A. Gaziev
中科院分区：
文献类型：
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作者：
E. V. Evdokimovskii;M. Patrushev;T. Ushakova;A. Gaziev
在暴露于 X 射线后，小鼠血细胞中线粒体 DNA (mtDNA) 拷贝数的变化及其转录的变化被追踪；此外，这些小鼠的血清中还记录了细胞外线粒体DNA片段。通过实时聚合酶链反应 (RT-PCR)，在 1 Gy X 射线照射的小鼠的血细胞中检测到暴露一小时后 mtDNA 拷贝数急剧增加。 mtDNA 拷贝数的增加被认为是小鼠因部分 mtDNA 分子受到辐射损伤而产生的补偿反应的结果。暴露于 10 Gy 剂量的 X 射线辐射导致小鼠血细胞中 mtDNA 复制在辐射后三小时内停止。同时，RT-PCR检测表明，在相同的辐射剂量下，线粒体nd6基因的mtRNA相对于核基因gapdh mRNA的数量发生增加。这表明，尽管与核DNA（nDNA）相比，mtDNA受到更多损伤，但在暴露于10 Gy后，小鼠血细胞线粒体中的转录过程仍在继续，而受损nDNA的转录则被抑制。小鼠接受10 Gy照射后，血清中记录到1841 bp的mtDNA片段。因此，在暴露于X射线的小鼠的血细胞中，mtDNA的拷贝数及其转录发生急剧变化，并且在血清中观察到大的mtDNA片段。
Changes in the number of mitochondrial DNA (mtDNA) copies and alterations in its transcription were followed in the blood cells of mice after their exposure to X-rays; also, extracellular mtDNA fragments were registered in the blood serum of these mice. By a real time polymerase chain reaction (RT-PCR), a sharp increase in mtDNA copy number one hour after the exposure was detected in the blood cells of 1 Gy X-ray irradiated mice. This increase in mtDNA copies is considered as a result of a compensatory reaction developed in mice in response to the radiation damage in a part of mtDNA molecules. Exposure to X-ray radiation at a dose of 10 Gy resulted in an arrest of mtDNA replication in mouse blood cells within three hours after irradiation. At the same time, RT-PCR assays showed that at the same radiation dose, an increase in the number of mtRNA of the mitochondrial nd6 gene relative to mRNA of the nuclear gene gapdh occurs. This indicates that, although mtDNA is more damaged in comparison with nuclear DNA (nDNA), the process of transcription continues in the blood cell mitochondria of mice after their exposure to 10 Gy, whereas the transcription of damaged nDNA is arrested. After the irradiation of mice with 10 Gy, mtDNA fragments of 1841 bp were registered in the blood serum. Thus, in the blood cells of mice exposed to X-rays, the copy number of mtDNA and its transcription are sharply changed, and large mtDNA fragments are observed in the blood serum.