On the regeneration and redifferentiation of mammalian striated muscle
On the regeneration and redifferentiation of mammalian striated muscle
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DOI：
10.1002/jmor.1051000103
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发表时间：
1957-01-01
期刊：
影响因子：
--
通讯作者：
GODMAN, GABRIEL CHARLES
中科院分区：
文献类型：
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作者：
GODMAN, GABRIEL CHARLES
在兔肌肉损伤愈合（缺血性梗塞和热损伤）以及成熟大鼠肌肉的组织培养中研究了成熟横纹骨骼肌的再生。关注分离细胞的起源、生肌成分的转化以及成肉细胞的细胞分化；描述了体内和体外肌肉再生过程中发生的细胞形式。肌肉损伤后出现的大量巨噬细胞中的许多来自肌内膜的固定组织细胞，但游离巨噬细胞也源自部分退化的肌纤维节段的幸存细胞核和肌浆，其收缩元件已在体外来自成肌细胞。这种部分退化的肌纤维的幸存元素也会产生细长带或滑移或不连续细胞形式的肌母细胞，以及肌芽（肌源性巨细胞）。然而，在体内，成肉细胞通常起源于完整肌纤维末端的连续宽锥形带。游离的巨噬细胞，无论其来源如何，都可以通过前者吸收活体染料（台盼蓝）来与成肉细胞和芽区分开，而后者则不能。肉母细胞生长迅速，其细胞核无丝分裂增殖。在它们的细胞质分化中，进行性前原纤维、原纤维、交叉纹状和成熟阶段被识别，并且它们的特征被详细描述。核外观的变化伴随着这些发展。受伤肌肉的恢复虽然广泛，但永远不会完全；列举了影响再生的解剖学条件。本研究强调了成熟横纹骨骼肌纤维增殖、再分化和调节的巨大内在能力。
The regeneration of mature striated skeletal muscle is studied in vivo in healing lesions of rabbit muscle (ischaemic infarcts and thermal injuries), and in tissue cultures of mature rat muscle. Attention is given to the origin of isolated cells, the transformation of myogenic elements, and to cytodifferentiation of the sarcoblasts; the cell forms occurring in the course of muscle regeneration in vivo and in vitro are described. Many of the numerous macrophagic cells which appeared after muscle injury are derived from the fixed histiocytes of the endomysium, but free macrophagic cells also originate from the surviving nuclei and sarcoplasm of partly degenerated muscle fiber segments whose contractile elements have succumbed, and in vitro, from sarcoblasts. The surviving elements of such partly degenerated muscle fibers also give rise to sarcoblasts in the form of slender straps or slips or discontinuous cells, and to muscle buds (myogenic giant cells). In vivo, however, sarcoblasts usually originate as continuous wide tapering ribbons from the ends of intact muscle fibers. Free macrophagic cells, whatever their origin, can be distinguished from the sarcoblasts and buds by uptake of vital dye (trypan blue) by the former but not the latter. The sarcoblasts grew rapidly, their nuclei proliferating amitotically. In their cytoplasmic differentiation, progressive prefibril, fibril, cross-striated and maturation stages are recognized and their characteristics detailed. Changing nuclear appearances accompany these developments. Restitution of injured muscle although extensive is never complete; anatomical conditions affecting regeneration are enumerated. The great intrinsic capacity of mature striated skeletal muscle fibers for proliferation, redifferentiation, and modulation is emphasized in this study.