The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize
The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize
复制标题
DOI：
10.1007/s11103-018-0751-8
复制
发表时间：
2018-06
影响因子：
5.1
通讯作者：
Xiaoming Yu;Xinchao Meng;Yutong Liu;Ning Li;Ai Zhang;Tian-Jing Wang;Lili Jiang;J. Pang;
中科院分区：
文献类型：
--
作者：
Xiaoming Yu;Xinchao Meng;Yutong Liu;Ning Li;Ai Zhang;Tian-Jing Wang;Lili Jiang;J. Pang;
玉米染色质重塑蛋白ZmCHB 101在渗透胁迫反应中起着重要作用。ZmCHB 101控制着胁迫应答基因转录起始位点周围的核小体密度，干旱和渗透胁迫是严重制约作物产量的经常性条件。在模式植物拟南芥（Arabidopsis thalianasgests）中积累的证据表明，SWI/SNF染色质重塑复合物的核心组分在非生物胁迫响应中起着重要作用。然而，玉米SWI/SNF染色质重塑复合物如何在渗透胁迫和干旱胁迫反应中发挥作用仍不清楚。在这里，我们表明ZmCHB 101，A. thalianaSWI 3D在玉米的渗透胁迫和脱水胁迫反应中起重要作用。ZmCHB 101-RNA干扰（RNAi）转基因植株表现出对渗透胁迫、盐胁迫和干旱胁迫敏感的表型。全基因组RNA测序分析表明，ZmCHB 101影响渗透胁迫响应基因的转录表达格局。有趣的是，ZmCHB 101控制着必需的应激反应基因转录起始位点周围的核小体密度。此外，我们确定ZmCHB 101与RNA聚合酶II（RNAPII）在体内，是一个先决条件，正确的占用RNAPII的近端区域的转录起始位点的应激反应基因。总之，我们的研究结果表明，ZmCHB 101影响基因表达的重塑染色质状态和控制RNAPII occupancy在玉米渗透胁迫下。
Key messageThe maize chromatin remodeler ZmCHB101 plays an essential role in the osmotic stress response. ZmCHB101 controls nucleosome densities around transcription start sites of essential stress-responsive genes.AbstractDrought and osmotic stresses are recurring conditions that severely constrain crop production. Evidence accumulated in the model plantArabidopsis thalianasuggests that core components of SWI/SNF chromatin remodeling complexes play essential roles in abiotic stress responses. However, how maize SWI/SNF chromatin remodeling complexes function in osmotic and drought stress responses remains unknown. Here we show that ZmCHB101, a homolog ofA. thalianaSWI3D in maize, plays essential roles in osmotic and dehydration stress responses. ZmCHB101-RNA interference (RNAi) transgenic plants displayed osmotic, salt and drought stress-sensitive phenotypes. Genome-wide RNA-sequencing analysis revealed that ZmCHB101 impacts the transcriptional expression landscape of osmotic stress-responsive genes. Intriguingly, ZmCHB101 controls nucleosome densities around transcription start sites of essential stress-responsive genes. Furthermore, we identified that ZmCHB101 associates with RNA polymerase II (RNAPII) in vivo and is a prerequisite for the proper occupancy of RNAPII on the proximal regions of transcription start sites of stress-response genes. Taken together, our findings suggest that ZmCHB101 affects gene expression by remodeling chromatin states and controls RNAPII occupancies in maize under osmotic stress.