UBIQUITIN-SPECIFIC PROTEASE16 interacts with a HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 and modulates cadmium tolerance
UBIQUITIN-SPECIFIC PROTEASE16 interacts with a HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 and modulates cadmium tolerance
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DOI：
10.4161/psb.25680
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发表时间：
2013-07
影响因子：
2.9
通讯作者：
Jinfeng Zhao;Huapeng Zhou;Xueyong Li
中科院分区：
文献类型：
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作者：
Jinfeng Zhao;Huapeng Zhou;Xueyong Li
蛋白质的泛素化和去泛素化是两个分别由泛素连接酶和去泛素化酶催化的可逆过程。在拟南芥中，发现了大量的泛素连接酶的底物，而只有少数的去泛素化酶的目标被确定。最近，我们报道了一种功能性的泛素特异性蛋白酶16（UBP 16）通过正向调节质膜Na+/H+反向转运活性和至少部分调节丝氨酸羟甲基转移酶1（SHM 1）的稳定性和活性参与耐盐性。在这里，我们报告说，UBP 16与重金属相关的异戊二烯化植物蛋白27（HIPP 27），金属伴侣含有一个预测的重金属相关的结构域，这已被报道发挥了重要作用，镉解毒相互作用。同时，ubp 16突变体表现出更敏感的镉比野生型。综上所述，HIPP 27可能是UBP 16在镉响应中的另一个靶点。
Protein ubiquitination and deubiquitination are two reversible processes catalyzed by ubiquitin ligases and deubiquitinating enzymes, respectively. In Arabidopsis, lots of substrates of ubiquitin ligases were found, whereas only a few targets of deubiquitinating enzymes were identified. Recently, we reported that a functional UBIQUITIN-SPECIFIC PROTEASE16 (UBP16) was involved in salt tolerance through positively regulating plasma membrane Na+/H+ antiport activity and at least partially modulating SERINE HYDROXYMETHYLTRANSFERASE1 (SHM1) stability and activity. Here, we report that UBP16 interacts with HEAVY METAL ASSOCIATED ISOPRENYLATED PLANT PROTEIN27 (HIPP27), a metallochaperone containing a predicted heavy-metal-associated domain, which has been reported to play an important role in cadmium detoxification. Meanwhile, the ubp16 mutant showed more sensitive to cadmium than wild-type. Taken together, HIPP27 may be another target of UBP16 in cadmium response.