SWCNT suppress inflammatory mediator responses in human lung epithelium in vitro
SWCNT suppress inflammatory mediator responses in human lung epithelium in vitro
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DOI：
10.1016/j.taap.2008.10.015
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发表时间：
2009-02-01
影响因子：
3.8
通讯作者：
Oostingh, Gertie Janneke
中科院分区：
文献类型：
--
作者：
Herzog, Eva;Byrne, Hugh J.;Oostingh, Gertie Janneke
单壁碳纳米管由于各种潜在的应用而获得了巨大的普及，这将最终导致人类和环境对这些纳米颗粒的暴露增加。本研究旨在评价永生化和原代人肺上皮细胞（A549和NHBE）对单壁碳纳米管样品（SWCNT）的炎症反应。特别关注肺表面活性物质对颗粒毒性的介导作用。将分散在细胞培养基中的SWCNT的毒性与分散在二棕榈酰磷脂酰胆碱（DPPC，肺衬里液的主要成分）中的纳米管的毒性进行比较。暴露进行6至48小时，后一个时间点显示出最显著的反应。此外，在存在促炎刺激因子-turnout necrosis factor-α（TNF-α）的情况下进行暴露，以模拟受刺激细胞的暴露，如感染期间发生的那样。评价的终点包括细胞活力、增殖和炎症介质如白细胞介素（IL）-8，IL-6，TNF-α和巨噬细胞趋化蛋白-1（MCP-1）的分析。青石棉被列为一种特性良好的有毒纤维对照品。该研究的结果表明，HiPco SWCNT样品抑制A549和NHBE细胞的炎症反应。对于TNF-α刺激的细胞也是如此。DPPC的使用提高了SWCNT在A549培养基中的分散程度，从而导致颗粒毒性增加，然而，它并未显示出改变NHBE细胞反应。
Single-walled carbon nanotubes have gained enormous popularity due to a variety of potential applications which will ultimately lead to increased human and environmental exposure to these nanoparticles. This study was carried out in order to evaluate the inflammatory response of immortalised and primary human lung epithelial cells (A549 and NHBE) to single-walled carbon nanotube samples (SWCNT). Special focus was placed on the mediating role of lung surfactant on particle toxicity. The toxicity of SWCNT dispersed in cell culture medium was compared to that of nanotubes dispersed in dipalmitoylphosphatidylcholine (DPPC, the main component of lung lining fluid). Exposure was carried out for 6 to 48 h with the latter time-point showing the most significant responses. Moreover, exposure was performed in the presence of the pro-inflammatory stimulus turnout necrosis factor-alpha (TNF-alpha) in order to mimic exposure of stimulated cells, as would occur during infection. Endpoints evaluated included cell viability, proliferation and the analysis of inflammatory mediators such as interleukin (IL)-8, IL-6, TNF-alpha and macrophage chemoattractant protein-1 (MCP-1). Crocidolite asbestos was included as a well characterised, toxic fibre control. The results of this study showed that HiPco SWCNT samples suppress inflammatory responses of A549 and NHBE cells. This was also true for TNF-alpha stimulated cells. The use of DPPC improved the degree of SWCNT dispersion in A549 medium and in turn, leads to increased particle toxicity, however, it was not shown to modify NHBE cell responses.