Nestmate recognition cues in the honey bee: Differential importance of cuticular alkanes and alkenes
Nestmate recognition cues in the honey bee: Differential importance of cuticular alkanes and alkenes
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DOI：
10.1093/chemse/bji040
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发表时间：
2005-07-01
期刊：
影响因子：
3.5
通讯作者：
Turillazzi, S
中科院分区：
文献类型：
--
作者：
Dani, FR;Jones, GR;Turillazzi, S
在社会性昆虫中，识别来自外星人的巢友是基于嗅觉线索，许多研究表明，这些线索包含在昆虫角质层的脂质层中。这些脂质通常是数十种化合物的复杂混合物，其中脂族烃通常是主要组分。这里描述的实验测试是否通过补充天然存在的烷烃和烯烃在蜜蜂的角质层配置文件的人为变化影响的行为的巢守卫。将化合物以微克量施用于活的觅食蜂，蜜蜂返回到它们的蜂巢入口，在那里观察守卫蜂的行为。以这种方式，我们比较了单一烯烃与单一烷烃的效果;烯烃混合物与烷烃混合物的效果;以及从表皮脂质分离的全烷烃馏分与烯烃馏分的效果。只有一个例外（n-C-19和（Z）9-C-19之间的比较），在所有实验中，用烯烃处理的蜜蜂比用烷烃处理的蜜蜂受到更强烈的攻击。这使我们得出结论，修改的天然化学配置文件与两个不同类别的化合物具有不同的效果接受，并表明这可能对应于一个不同的重要性，在识别签名。
In social insects, recognition of nestmates from aliens is based on olfactory cues, and many studies have demonstrated that such cues are contained within the lipid layer covering the insect cuticle. These lipids are usually a complex mixture of tens of compounds in which aliphatic hydrocarbons are generally the major components. The experiments described here tested whether artificial changes in the cuticular profile through supplementation of naturally occurring alkanes and alkenes in honeybees affect the behaviour of nestmate guards. Compounds were applied to live foragers in microgram quantities and the bees returned to their hive entrance where the behaviour of the guard bees was observed. In this fashion we compared the effect of single alkenes with that of single alkanes; the effect of mixtures of alkenes versus that of mixtures of alkanes and the whole alkane fraction separated from the cuticular lipids versus the alkene fraction. With only one exception (the comparison between n-C-19 and (Z)9-C-19), in all the experiments bees treated with alkenes were attacked more intensively than bees treated with alkanes. This leads us to conclude that modification of the natural chemical profile with the two different classes of compounds has a different effect on acceptance and suggests that this may correspond to a differential importance in the recognition signature.