Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
Detection and Structural Characterization of SFAHFA Homologous Series in Mouse Colon Contents by LTQ-Orbitrap-MS and Their Implication in Influenza Virus Infection
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DOI：
10.1021/jasms.1c00138
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发表时间：
2021-06-25
影响因子：
3.2
通讯作者：
Hui, Shu-Ping
中科院分区：
文献类型：
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作者：
Gowda, Siddabasave Gowda B.;Gowda, Divyavani;Hui, Shu-Ping
羟基脂肪酸酯（FAHFA）是哺乳动物体内一类具有重要生理功能的新型内源性脂质。我们之前向该家族引入了一种新型脂质，称为羟基脂肪酸的短链脂肪酸酯（SFAHFA），其分支特异于长链脂肪酸（>= C20）的C2碳。在这项研究中，我们在小鼠结肠内容物中发现了一系列同源的SFAHFA，其包含用短链脂肪酸（C2-C5）酯化的C16-C26羟基脂肪酸。检测到的SFAHFA，其特征在于高分辨率质谱与MSn分析。通过与间氯过氧苯甲酸的环氧化反应和MSn分析确定了单不饱和SFAHFA的双键位置。此外，用流感A/波多黎各/8/34（H1N1; PR 8）病毒感染的小鼠的结肠内容物中SFAHFA浓度的测量显示，与天然对照相比，它们的水平显著增加。羟基脂肪酸和SFAHFA之间观察到很强的相关性。检测、表征和分析这些与大流行性H1N1; PR 8流感病毒相关的新SFAHFA水平将有助于深入研究它们的功能和代谢。
Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with promising physiological functions in mammals. We previously introduced a new type of lipids to this family called short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs), branching specific to the C2 carbon of a long-chain fatty acid (>= C20). In this study, we discovered a homologous series of SFAHFAs comprising C16-C26 hydroxy fatty acids esterified with short-chain fatty acids (C2-C5) in mouse colon contents. The detected SFAHFAs were characterized by high-resolution mass spectrometry with MSn analysis. The double-bond position of monounsaturated SFAHFAs was determined by the epoxidation reaction of samples with m-chloroperoxybenzoic acid and their MSn analysis. Further, the measurement of SFAHFA concentration in the colon contents of mice infected with influenza A/Puerto Rico/8/34 (H1N1; PR8) virus revealed a significant increase in their levels compared to native control. A strong correlation was observed between hydroxy fatty acid and SFAHFAs. Detection, characterization, and profiling of these new SFAHFA levels in relation with pandemic H1N1; PR8 influenza virus will contribute to the in-depth study of their function and metabolism.