Enhancement of in vitro capillary tube formation by substrate nanotopography
Enhancement of in vitro capillary tube formation by substrate nanotopography
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DOI：
10.1002/adma.200702487
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发表时间：
2008-01-07
影响因子：
29.4
通讯作者：
Langer, Robert
中科院分区：
文献类型：
--
作者：
Bettinger, Christopher J.;Zhang, Zhitong;Langer, Robert
内皮祖细胞在具有线光栅几何结构的纳米基质上培养，与平面基质相比，内皮祖细胞通过排列和拉长的形态、减少的增殖和增加的迁移来对这些基质做出反应。细胞还形成超细胞带结构，加入基质后毛细血管形成增强。这些结果表明，底物纳米形貌可以用于体外形成有组织的血管结构。以250倍放大率拍摄的图像。
Endothelial progenitor cells are cultured on nanotopographic substrates with a line-grating geometry and respond to these substrates through aligned and elongated morphology, reduced proliferation, and increased migration relative to flat substrates. The cells also form supercellular band structures, which lead to enhanced capillary tube formation upon the addition of matrigel. These results suggest that substrate nanotopography can be used to create organized vascular structures in vitro. Image taken at 250 x magnification.